# US 10,246,710 B2 · Double strand RNA as molecular biopesticides for RNA interference through feeding in the hemipteran invasive insect pest, brown marmorated stink bug > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,246,710 B2 - Document ID: US10246710B2 - Status: Granted - Kind: B2 - Application number: 15/657,646 - Filed: 2017-07-24 - Published: 2018-01-25 - Granted: 2019-04-02 - Earliest claimed: 2016-07-25 - Assignees: The United States of America as represented by the Secretary of Agriculture - Applicants: The United States of America, as Represented By the Secretary of Agriculture - Inventors: Saikat Kumar B. Ghosh; Dawn E. Gundersen-Rindal - Web page: https://patentgrep.com/patent/US10246710B2 ## CPC - A01N 63/60: Isolated nucleic acids - A01N 37/46: N-acyl derivatives - A01N 57/16: containing heterocyclic radicals - C12N 15/113: Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} - C12N 15/8218: {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]} - C12N 15/8286: {for insect resistance} - C12N 2310/14: interfering nucleic acids [NA] - Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract The present disclosure provides compositions and methods of delivering double strand ribonucleic acid (dsRNA) to control the insect pest, the brown marmorated stink bug. Several dsRNA species that target genes are disclosed herein that can be used individually or in combination. The dsRNA species can be used to induce RNA-mediated interference (RNAi) to decrease target gene expression. RNAi can be used alone, or in combination with other pest control measures to target the brown marmorated stink bug. ## Claims ### Claim 1 A double-stranded ribonucleic acid (dsRNA) comprising a sense region with at least 95% sequence identity to any one of SEQ ID NOs: 56, 66, 67, or 68 and an antisense region comprising a second sequence complementary to the sense region, wherein said dsRNA is capable of inducing ribonucleic acid interference (RNAi) in an insect. ### Claim 2 (depends on 1) The dsRNA of claim 1, wherein the sense region has at least 99% or 100% sequence identity to any one of SEQ ID NOs: 56, 66, 67, or 68. ### Claim 3 (depends on 1) The dsRNA of claim 1, wherein the dsRNA is distributed throughout at least part of a living plant material's vascular tissues. ### Claim 4 (depends on 3) The dsRNA of claim 3, wherein the living plant material is a fruit, vegetable, stem or leaf. ### Claim 5 (depends on 1) The dsRNA of claim 1, wherein the dsRNA is expressed in a bacterial or yeast cell. ### Claim 6 (depends on 1) The dsRNA of claim 1, wherein the dsRNA is expressed in a transgenic plant cell. ### Claim 7 (depends on 1) The dsRNA of claim 1, wherein the insect is a brown marmorated stink bug. ### Claim 8 (depends on 1) A method of controlling H. halys comprising applying one or more dsRNA molecules of claim 1 to a living plant material such that the one or more dsRNA molecules are taken up and distributed by the vascular tissue of the living plant material and allowing the one or more insects to ingest an effective amount of the one or more dsRNA molecules to induce RNAi, thereby controlling the one or more insects. ### Claim 9 (depends on 8) The method of claim 8, wherein the one or more dsRNA molecules comprise a first dsRNA molecule and a second dsRNA molecule, wherein the first dsRNA molecule comprises a sense region with at least 95% sequence identity to SEQ ID NO: 56 and an antisense region comprising a second sequence complementary to the sense region and wherein the second dsRNA molecule comprises a sense region with at least 95% sequence identity to SEQ ID NO: 68 and an antisense region comprising a second sequence complementary to the sense region. ### Claim 10 A method of controlling H. halys comprising the steps of: a) providing a living plant material containing at least one double-strand RNA (dsRNA) wherein the at least one dsRNA molecule comprises a sense region with at least 95% sequence identity to SEQ ID NOs: 56, 66, 67, or 68 and an antisense region comprising a second sequence complementary to the sense region, wherein the at least one dsRNA molecule is distributed throughout at least part of the living plant material's vascular tissues and wherein the living plant material does not contain genetic information allowing for the production of the at least one double strand dsRNA molecule; b) allowing the insect to ingest a sufficient amount of the at least one dsRNA molecule, by feeding on the plant material, to interfere with the production of at least one protein targeted by the at least one dsRNA molecule, thereby inducing RNAi in the insect, and; c) controlling the insect via RNAi. ### Claim 11 (depends on 10) The method of claim 10, wherein the living plant material is a fruit, vegetable, stem or leaf. ### Claim 12 (depends on 11) The method of claim 11, wherein the living plant material is a green bean. ### Claim 13 (depends on 10) The method of claim 10, wherein the at least one dsRNA molecule comprises two dsRNA molecules wherein the first dsRNA molecule comprises a sense region with at least 95% sequence identity to SEQ ID NO: 56 and an antisense region comprising a second sequence complementary to the sense region and wherein the second dsRNA molecule comprises a sense region with at least 95% sequence identity to SEQ ID NO: 68 and an antisense region comprising a second sequence complementary to the sense region.