# US 10,246,699 B2 · Microparticle separation apparatus assembly comprising multiple separable panels

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- Number: US 10,246,699 B2
- Document ID: US10246699B2
- Status: Granted
- Kind: B2
- Application number: 15/564,371
- Filed: 2015-05-18
- Published: 2018-05-10
- Granted: 2019-04-02
- Earliest claimed: 2015-04-08
- Assignees: Inje University Industry-Academic Cooperation Foundation
- Applicants: Inje University Industry-Academic Cooperation Foundation
- Inventors: Ki Ho Han; Hyung Seok Cho
- Web page: https://patentgrep.com/patent/US10246699B2

## CPC

- C12N 13/00: Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules}
- B03C 1/025: High gradient magnetic separators
- B03C 1/288: {disposed at the outer circumference of a recipient}
- C12M 1/12: with sterilisation, filtration or dialysis means
- C12M 1/42: Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
- C12M 47/02: {Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02)}
- C12Q 1/24: Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
- G01N 33/50: Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing (measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q)
- B01L 2200/0652: Sorting or classification of particles or molecules
- B01L 2300/0851: Bottom walls
- B01L 2400/043: magnetic forces
- B03C 2201/18: Magnetic separation whereby the particles are suspended in a liquid
- B03C 2201/26: for use in medical or biological applications
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B03C: MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS (separating isotopes B01D59/00; combinations of magnetic or electrostatic separation with separation of solids by other means B03B, B07B)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention relates to a microparticle separation apparatus assembly comprising multiple separable panels, more specifically, to a microparticle separation apparatus assembly having a novel structure, comprising: a first panel comprising a magnetic microstructure for applying magnetophoretic force to microparticles; and a second panel, which can be separated from the first panel and through which a sample comprising microparticles passes, wherein the first panel comprising the magnetic microstructure can be recycled.

## Claims

### Claim 1

A microparticle separation apparatus assembly comprising:
a first panel comprising a magnetic microstructure;
a second panel detachable from the first panel and including a microchannel structure through which the sample passes; and
a thin film portion between the second panel and the first panel, the thin film portion having a thickness of 50 um or less and made of PET, PI, PE, PP, PMMA or glass,
wherein the magnetic microstructures included in the first panel include a pattern formed in a direction perpendicular to the direction in which the sample is introduced.

### Claim 2 (depends on 1)

The microparticle separation device assembly according to claim 1, wherein the magnetic microstructures included in the first panel are inclined at an angle with respect to a flow direction of cells in the second panel.

### Claim 3 (depends on 1)

The microparticle separation device assembly of claim 1, further comprising a coupling portion for coupling the first panel and the second panel.

### Claim 4 (depends on 3)

The microparticle separation device assembly of claim 3, wherein the coupling portion comprises a vacuum applying portion, or a magnet.

### Claim 5 (depends on 3)

The fine particle separator assembly of claim 3, wherein the coupling portion includes concave and convex portions formed in correspondence with the first panel and the second panel.

### Claim 6 (depends on 1)

The apparatus of claim 1, wherein the first panel comprises n first 1-1 panels, a 1-2 panel, and a first-n panel are connected.

### Claim 7 (depends on 1)

The apparatus of claim 1, wherein the second panel comprises n second-1 panels, second-2 panels, and a second-n panel are connected.

### Claim 8 (depends on 6)

The fine particle separation apparatus according to claim 6, wherein the first-n panel is in contact with the second-n panel and the thin film part.

### Claim 9 (depends on 8)

The microparticle separation device assembly of claim 8, wherein the magnetic microstructures included in the first-n panel are perpendicular to the sample flow direction.

### Claim 10 (depends on 1)

The microparticle separation device assembly of claim 1, wherein the first panel is located on top of the second panel.

### Claim 11 (depends on 1)

The microparticle separation device assembly of claim 1, wherein the first panel is located below the second panel.

### Claim 12 (depends on 1)

The microparticle separation device assembly of claim 1, wherein the first panel and the second panel further comprise an alignment portion for aligning the sample fluid channel with the magnetic microstructure.

### Claim 13 (depends on 7)

The fine particle separation apparatus according to claim 7, wherein the first-n panel is in contact with the second-n panel and the thin film part.