Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
AssigneeNovozymes, Inc.
InventorsSuchindra Maiyuran, Randall Kramer, Paul Harris
Life of this patent
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Abstract
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims39
A DNA construct or recombinant expression vector comprising an isolated polynucleotide comprising a nucleotide sequence that encodes a GH61 polypeptide having cellulolytic enhancing activity, wherein the isolated polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from:
- (a) a GH61 polypeptide having at least 95% sequence identity to amino acids 20 to 223 of SEQ ID NO:
- 8;
- and
- (b) a GH61 polypeptide encoded by a polynucleotide having at least 99% sequence identity to nucleotides 58 to 974 of SEQ ID NO:
- 7, or a GH61 polypeptide encoded by the cDNA of nucleotides 58 to 974 of SEQ ID NO:
- 7.
A DNA construct comprising a nucleic acid encoding a signal peptide comprising amino acids acids 1 to 19 of SEQ ID NO:
- 8, wherein the nucleic acid is operably linked to a heterologous nucleic acid encoding a protein.
A recombinant host cell comprising a nucleic acid encoding a protein, wherein the nucleic acid is operably linked to an isolated polynucleotide encoding a signal peptide comprising amino acids 1 to 19 of SEQ ID NO:
- 8, wherein the nucleic acid is foreign to the polynucleotide encoding the signal peptide.
A transgenic plant, plant part or plant cell transformed with a polynucleotide encoding a GH61 polypeptide having cellulolytic enhancing activity, wherein the polypeptide comprises a signal peptide directing the polypeptide into the secretory pathway, and wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from:
- (a) a GH61 polypeptide having at least 95% sequence identity to amino acids 20 to 223 of SEQ ID NO:
- 8;
- and
- (b) a GH61 polypeptide encoded by a polynucleotide having at least 99% sequence identity to nucleotides 58 to 974 of SEQ ID NO:
- 7, or a GH61 polypeptide encoded by the cDNA of nucleotides 58 to 974 of SEQ ID NO:
- 7.
A method for degrading a cellulosic material comprising:
- (i) treating the cellulosic material with an enzyme composition comprising a GH61 polypeptide having cellulolytic enhancing activity, and
- (ii) recovering the degraded cellulosic material;
- wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from:
- (a) a GH61 polypeptide having at least 95% sequence identity to amino acids 20 to 223 of SEQ ID NO:
- 8;
- and
- (b) a GH61 polypeptide encoded by a polynucleotide having at least 99% sequence identity to nucleotides 58 to 974 of SEQ ID NO:
- 7, or a GH61 polypeptide encoded by the cDNA of nucleotides 58 to 974 of SEQ ID NO:
- 7.
A method for producing a fermentation product, said method comprising:
- (i) saccharifying a cellulosic material with an enzyme composition comprising a GH61 polypeptide having cellulolytic enhancing activity;
- (ii) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product;
- and
- (ii) recovering the fermentation product from the fermentation;
- wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from:
- (a) a GH61 polypeptide having at least 95% sequence identity to amino acids 20 to 223 of SEQ ID NO:
- 8;
- and
- (b) a GH61 polypeptide encoded by a polynucleotide having at least 99% sequence identity to nucleotides 58 to 974 of SEQ ID NO:
- 7, or a GH61 polypeptide encoded by the cDNA of nucleotides 58 to 974 of SEQ ID NO:
- 7.
A method of fermenting a cellulosic material comprising:
- (i) fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition comprising a polypeptide having cellulolytic enhancing activity, wherein the fermenting of the cellulosic material produces a fermentation product, and
- (ii) recovering the fermentation product from the fermentation;
- wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from:
- (a) a GH61 polypeptide having at least 95% sequence identity to amino acids 20 to 223 of SEQ ID NO:
- 8;
- and
- (b) a GH61 polypeptide encoded by a polynucleotide having at least 99% sequence identity to nucleotides 58 to 974 of SEQ ID NO:
- 7, or a GH61 polypeptide encoded by the cDNA of nucleotides 58 to 974 of SEQ ID NO:
- 7.
Dependencies are inferred from the claim text.