# US 10,246,692 B2 · Polypeptides having lipase activity and polynucleotides encoding same

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- Number: US 10,246,692 B2
- Document ID: US10246692B2
- Status: Granted
- Kind: B2
- Application number: 14/412,872
- Filed: 2013-07-11
- Published: 2016-02-25
- Granted: 2019-04-02
- Earliest claimed: 2012-07-12
- Assignees: Novozymes A/S
- Applicants: Novozymes A/S
- Inventors: Robert Piotr Olinski; Preben Nielsen; Kim Borch; Anna Verena Reiser; Carsten Hoerslev Hansen; Lone Baunsgaard
- Web page: https://patentgrep.com/patent/US10246692B2

## CPC

- C12N 9/20: Triglyceride splitting, e.g. by means of lipase
- C11D 3/38627: {containing lipase}
- C11D 3/38681: {Chemically modified or immobilised enzymes}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL

## Abstract

The present invention relates to isolated polypeptides having lipase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

## Claims

### Claim 1

A composition comprising an isolated polypeptide having lipase activity and having at least 94% sequence identity to the sequence of amino acids 1 to 292 of SEQ ID NO: 2, and one or more anionic surfactants,
wherein the anionic surfactants are present at a level of from 0.1 to 60 wt %,
wherein the composition is formulated at a pH from 8.0 to 11, and
wherein the composition is more effective in removing lipid stains present at a surface in comparison with an equivalent composition lacking the lipase.

### Claim 2 (depends on 1)

The composition of claim 1, wherein the isolated polypeptide comprises or consists of amino acids 1 to 292 of SEQ ID NO: 2.

### Claim 3 (depends on 1)

The composition of claim 1, wherein the composition is selected from the group consisting of a laundry cleaning composition, a dishwashing cleaning composition, a hard-surface cleaning composition and a personal care cleaning composition.

### Claim 4 (depends on 1)

The composition of claim 1, wherein the composition is in the form of a liquid, a gel, a soap bar, a powder, a granulated solid, a tablet, or any combination thereof.

### Claim 5 (depends on 1)

The composition of claim 1, wherein the composition is effective in hydrolyzing a lipid at a temperature of from about 10° C. to 90° C.

### Claim 6 (depends on 1)

The composition of claim 1, further comprising one or more enzymes selected from the group consisting of hemicellulases, peroxidases, proteases, cellulases, xylanases, lipases, phospholipases, esterases, cutinases, pectinases, mannanases, pectate lyases, keratinases, reductases, oxidases, phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, malanases, β-glucanases, arabinosidases, hyaluronidase, chondroitinase, laccase, amylases, and mixtures thereof.

### Claim 7 (depends on 1)

The composition of claim 1, wherein the isolated polypeptide has greater stability than Thermomyces lanuginosus lipase in an equivalent composition.

### Claim 8 (depends on 1)

The composition of claim 1, further comprising one or more divalent cations selected from the group consisting of calcium (Ca2+), magnesium (Mg2+) and iron-II (Fe2+).

### Claim 9 (depends on 1)

The composition of claim 1, wherein the one or more anionic surfactants comprise a sulfate or sulphonate surfactant.

### Claim 10 (depends on 1)

The composition of claim 1, wherein the one or more anionic surfactants comprise an alkyl benzene sulfonate surfactant.

### Claim 11 (depends on 1)

The composition of claim 1, wherein the one or more anionic surfactants comprise alkyl polyglucoside or an alkyl alkoxylated alcohol.

### Claim 12 (depends on 1)

The composition of claim 1, wherein the composition is more effective in laundry washing detergent compared to an equivalent composition comprising Thermomyces lanuginosus lipase.

### Claim 13

A composition, comprising:
an isolated polypeptide having lipase activity, wherein the isolated polypeptide consists of a polypeptide that differs from the sequence of amino acids 1 to 292 of SEQ ID NO: 2 by at least 1 amino acid but by no more than 17 amino acids;
wherein one or more anionic surfactants are present at a level of from 1 to 50 wt %, and
wherein the composition is formulated at a pH of between 8 and 10.5.