# US 10,246,675 B2 · Biochemical cartridge, and biochemical cartridge and cartridge holder set > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,246,675 B2 - Document ID: US10246675B2 - Status: Granted - Kind: B2 - Application number: 14/762,301 - Filed: 2014-01-27 - Published: 2015-12-17 - Granted: 2019-04-02 - Earliest claimed: 2013-01-31 - Assignees: Hitachi High-Technologies Corporation - Applicants: Hitachi High-Technologies Corporation - Inventors: Ryusuke Kimura; Hiromi Yamashita; Motohiro Yamazaki; Taro Nakazawa - Web page: https://patentgrep.com/patent/US10246675B2 ## CPC - C12M 23/42: {Integrated assemblies, e.g. cassettes or cartridges} - B01L 3/50273: {characterised by the means or forces applied to move the fluids} - B01L 3/502738: {characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746)} - C12M 21/18: {Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes} - C12M 23/36: {Means for collection or storage of gas; Gas holders} - F16K 7/17: the diaphragm being actuated by fluid pressure - F16K 31/126: the fluid acting on a diaphragm, bellows, or the like (F16K31/145, F16K31/165, F16K31/365, F16K31/385 take precedence) - G01N 35/08: using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis - B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions - B01L 2300/0874: Three dimensional network - B01L 2300/123: Flexible; Elastomeric - B01L 2400/0481: squeezing of channels or chambers - B01L 2400/0655: pinch valves - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - F16K: VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING {(devices for emptying and evacuating the excess liquid in valves or conduits F16L55/07)} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A cartridge sealed from ambient air is used to send a liquid without causing the liquid to contact the fluid inside the cartridge. Inside of the cartridge 1 sealed from ambient air are provided chambers that send and receive reagents, and an elastic body membrane 51 is attached to the bottom surface. The cartridge main body 51 does not have a groove or anything else that becomes a channel, and the membrane 51 is not bonded to portions that become channels. A channel, not formed in a normal state, is formed upon deforming the membrane 51 under air pressure in the unbonded portion, and the fluid is moved inside. A valve function is provided at an inlet of each chamber, and the fluid is internally moved in any direction with channel deformation. ## Claims ### Claim 1 A cartridge for sending a liquid inside a sealed space, the cartridge comprising: a cartridge main body having a top surface and a bottom surface substantially in one plane; a plurality of chambers internally provided in the cartridge main body that accept the liquid, including a first chamber having a first opening, a second chamber having a second opening and a third opening, and a third chamber having a fourth opening; and an elastic body having substantially planar top and bottom surfaces wherein one of the surfaces of the elastic body is attached to only first portions of the bottom surface of the cartridge main body among the first portions and second portions of the bottom surface of the cartridge main body, wherein the elastic body undergoes deformation with changes in externally applied pressure, wherein the elastic body is configured to close the bottom surface side of the plurality of chambers, wherein a part of the second portions of the bottom surface of the cartridge main body is between the first opening of the first chamber and the second opening of the second chamber and another part of the second portions of the bottom surface of the cartridge main body is between the third opening of the second chamber and the fourth opening of the third chamber, wherein the elastic body only deforms away from the substantially planar bottom surface of the cartridge main body at the second portions of the bottom surface of the cartridge main body wherein a flow channel is formed after the elastic body is deformed, wherein in a state without externally applied pressure causing deformation of the elastic body, substantially an entirety of the one surface of the elastic body contacts the bottom surface of the cartridge main body in the one plane, wherein at least the first chamber and the second chamber initially hold a reagent, and wherein a film is attached to the top surface of the cartridge main body covering each of the first chamber, the second chamber, and the third chamber. ### Claim 2 (depends on 1) A biochemical cartridge system comprising the cartridge of claim 1, and a cartridge holder on which the cartridge is set, wherein the cartridge holder has a channel shape formed therein, and the channel is formed in conformity with the channel shape by the deformation of the elastic body. ### Claim 3 (depends on 2) The biochemical cartridge system according to claim 2, wherein the channel has at least one of a height and a width that allows the elastic body to contact the channel shape by the deformation of the elastic body. ### Claim 4 (depends on 1) A biochemical cartridge system comprising the cartridge of claim 1, and a cartridge holder on which the cartridge is set, wherein each of the first opening, second opening, third opening and fourth opening are respectively aligned over a first plunger, a second plunger, a third plunger, and a fourth plunger of the cartridge holder. ### Claim 5 (depends on 1) The cartridge according to claim 1, further comprising: channel grooves are disposed within at least one of the second portions of the bottom surface of the cartridge main body. ### Claim 6 (depends on 1) The cartridge according to claim 1, further comprising: a first channel, a second channel, a third channel, and a fourth channel disposed in the cartridge main body in respective communication with the first opening, the second opening, the third opening, and the fourth opening, wherein the each of the first channel, the second channel, the third channel, and the fourth channel extend downward from the chambers to the respective first opening, the second opening, the third opening, and the fourth opening.