# US 10,246,674 B2 · Light emitting diode photobioreactors and methods of use

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- Number: US 10,246,674 B2
- Document ID: US10246674B2
- Status: Granted
- Kind: B2
- Application number: 15/416,989
- Filed: 2017-01-26
- Published: 2017-05-18
- Granted: 2019-04-02
- Earliest claimed: 2015-07-29
- Assignees: Algal Research Center, LLC
- Applicants: Algal Research Center, LLC
- Inventors: David Punchard; Philippe Bois; Angela Cortina Burgueño
- Web page: https://patentgrep.com/patent/US10246674B2

## CPC

- C12M 21/02: {Photobioreactors (culturing algae A01G33/00, A01H4/001, C12N1/12)}
- C12M 23/58: {Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00)}
- C12M 27/00: {Means for mixing, agitating or circulating fluids in the vessel (by introduction of gas C12M29/06, C12M29/14, mixing in general or mixers per se B01F; mixing in apparatus for chemical or physical processes B01J)}
- C12M 29/06: {Nozzles; Sprayers; Spargers; Diffusers (per se B01F23/231, B01J19/26)}
- C12M 29/26: {Conditioning fluids entering or exiting the reaction vessel}
- C12M 31/10: {by light emitting elements located inside the reactor, e.g. LED or OLED}
- C12M 37/00: {Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination (C12M23/38 takes precedence; filtration in general and filters per se B01D24/00-B01D41/00; autoclaves B01J3/04; treatment of microorganisms with electrical or wave energy C12N13/00)}
- C12N 1/12: Unicellular algae; Culture media therefor (as new plants A01H13/00)
- G02B 6/0068: {Arrangements of plural sources, e.g. multi-colour light sources}
- G02B 6/0078: {Side-by-side arrangements, e.g. for large area displays}
- G02B 6/0085: {Means for removing heat created by the light source from the package}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS

## Abstract

A photobioreactor system and a process for its use is illustrated, whereby water and nutrients from multiple sources are balanced (mixed using aeration) to the specific requirements of the particular photosynthetic organism strain used, sterilized, further mixed to balance the system and seeded with the photosynthetic microorganism, e.g. microalgae (dilution of a concentrated stock or added to an existing algal biomass). In accordance with such an embodiment, the algal biomass is then grown for a most efficient number of hours in a totally controlled environment where temperature (using aeration, an internal coil cooling system, or a combination thereof), pH (via CO2 delivery) and light delivery (using internal lighting directly inside the algal biomass) are optimized to the algal strain grown.

## Claims

### Claim 1

A cultivation system, comprising:
a firewall vessel configured to contain therein fluid, the firewall vessel being capable of communication with a source of sterilant configured to sterilize fluid originating from the firewall vessel;
a growth stage comprising one or more growth vessels that are capable of selective fluid communication with the firewall vessel,
the one or more growth vessels being configured to (1) receive fluid from the firewall vessel that has been sterilized with the sterilant, and (2) following removal of sufficient sterilant, support production of biomass by cultivation of photosynthetic microorganisms introduced to the one or more growth vessels;
a CO2 diffuser configured to deliver CO2 to the one or more growth vessels so as to affect pH levels in the one or more growth vessels;
at least one bubbler configured to provide airflow into the one or more growth vessels so as to circulate fluid disposed within the at least one growth vessel;
a light panel assembly immersed within a growth vessel of the growth stage; and
a fluidic pathway comprising one or more fluid channels and one or more valves, the fluidic pathway being switchable between
a first state that places fluid originating from the firewall vessel into communication with the one or more growth vessels, and
a second state that places the firewall vessel into fluid isolation from the one or more growth vessels.

### Claim 2 (depends on 1)

The cultivation system of claim 1, further comprising at least two bubblers, the at least two bubblers being constructed and arranged so as to give rise to alternating circulation of fluid disposed within the at least one growth vessel.

### Claim 3 (depends on 1)

The cultivation system of claim 1, wherein at least one growth vessel comprises an amount of photosynthetic microorganisms disposed therein.

### Claim 4 (depends on 1)

The cultivation system of claim 1, further comprising at least one temperature control coil in thermal communication with at least one growth stage vessel, the at least one temperature control coil configured to modulate the temperature of fluid within the at least one growth stage vessel.

### Claim 5 (depends on 1)

The cultivation system of claim 1, wherein said photosynthetic microorganisms are microalgae.

### Claim 6 (depends on 1)

The cultivation system of claim 1 wherein the at least one growth stage vessel comprises a source of photosynthetic microorganism nutrients.

### Claim 7 (depends on 1)

The cultivation system of claim 1, further comprising at least one stress vessel in fluid communication with a vessel of the growth stage so as to receive fluid from the vessel of the growth stage, the stress vessel optionally being in fluid communication with the firewall vessel, the stress vessel optionally comprising a lid constructed and arranged to maintain a positive pressure environment within the vessel.

### Claim 8 (depends on 7)

The cultivation system of claim 7, further comprising at least one fluidic pathway constructed and arranged to enable selective fluid communication from the at least one growth stage vessel to the at least one stress vessel.

### Claim 9 (depends on 7)

The cultivation system of claim 7, wherein the at least one stress vessel comprises at least one light panel assembly comprising at least one edge-fed light guide assembly, said edge-fed light guide assembly comprising at least one edge-fed two-sided light guide, at least one light-emitting diode (LED) strip, and at least one heatsink, wherein said at least one edge-fed two-sided light guide and said at least one LED strip are maintained in relative position by said heatsink, and wherein light emitted from said at least one LED strip is injected into the edge-fed two-sided light guide and is uniformly distributed from each side thereof.

### Claim 10 (depends on 7)

The cultivation system of claim 7, wherein the at least one stress vessel is in fluid communication with the firewall vessel such that the stress vessel receives fluid from the firewall vessel.

### Claim 11 (depends on 1)

The cultivation system of claim 1, wherein the sterilant comprises ozone.

### Claim 12 (depends on 1)

The cultivation system of claim 1, wherein fluid communication between the at least one vessel of the growth stage and the firewall vessel is arranged such that the at least one vessel of the growth stage receives fluid from the firewall vessel and the firewall vessel receives fluid from the at least one vessel of the growth stage.

### Claim 13 (depends on 1)

The cultivation system of claim 1, wherein said light panel assembly comprises at least one edge-fed two-sided light guide, at least one light-emitting diode (LED) strip, and at least one heatsink, wherein said at least one edge-fed two-sided light guide and said at least one LED strip are maintained in relative position by said heatsink and wherein light emitted from said at least one LED strip is injected into the two-sided light guide and is uniformly distributed from each side thereof.