# US 10,246,668 B2 · Proportioner-ready bioenzymatic concentrated cleaning product > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,246,668 B2 - Document ID: US10246668B2 - Status: Granted - Kind: B2 - Application number: 15/211,736 - Filed: 2016-07-15 - Published: 2016-12-15 - Granted: 2019-04-02 - Earliest claimed: 2011-08-24 - Assignees: Illinois Tool Works Inc. - Applicants: Illinois Tool Works Inc. - Inventors: Donald A. Cooper; Jonathan A. Long - Web page: https://patentgrep.com/patent/US10246668B2 ## CPC - A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46) - A01N 25/04: Dispersions, {emulsions, suspoemulsions, suspension concentrates} or gels (foams A01N25/16) - A01N 63/22: Bacillus - A61L 11/00: Methods specially adapted for refuse {(disintegrating medical waste B02C19/0075; disposal of medical waste B09B3/00)} - C11D 3/0068: {Deodorant compositions} - C11D 3/2086: {Hydroxy carboxylic acids-salts thereof} - C11D 3/222: {Natural or synthetic polysaccharides, e.g. cellulose, starch, gum, alginic acid or cyclodextrin} - C11D 3/386: Preparations containing enzymes {, e.g. protease or amylase} - C12N 1/20: Bacteria; Culture media therefor - C12N 3/00: Spore forming or isolating processes - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract A concentrated bioenzymatic cleaning product is provided which maintains a homogenous composition under static conditions and does not require shaking or mixing prior to dilution. The concentrated bioenzymatic cleaning product includes bacillus spores, a suspending and/or rheology modifying agent, an odor control agent, a pH adjuster, and water. The types and amounts of ingredients are selected to maintain the bacillus spores in suspension under static conditions, enabling the concentrated bioenzymatic cleaning product to be diluted using a proportioner system. ## Claims ### Claim 1 A bioenzymatic, concentrated cleaning product, comprising: 15 to 55% by weight bacillus spores, capable of generating extracellular enzymes selected from the group consisting of extracellular protease enzymes, extracellular urease enzymes, extracellular amylase enzymes, extracellular lypase enzymes, extracellular cellulase enzymes, and combinations thereof; a suspending and/ or rheology modifying agent; an odor control agent; sufficient pH adjuster to maintain a pH of about 5.0 to about 9.5; and water; wherein the 15-55% by weight bacillus spores have an average diameter of 5-50microns and remain suspended in the water under static conditions and the bioenzymatic, concentrated cleaning product is substantially free of organic solvents. ### Claim 2 (depends on 1) The bioenzymatic, concentrated cleaning product of claim 1, wherein water is the only solvent. ### Claim 3 (depends on 1) The bioenzymatic, concentrated cleaning product of claim 1. wherein the bacillus spores are present at 15 to 35% by weight. ### Claim 4 (depends on 1) The bioenzymatic, concentrated cleaning product of claim 1, wherein the suspending and/ or rheology modifying agent is selected from the group consisting of xanthan gum, guar gum, acrylate copolymers, celluloses, smectic clays, and combinations thereof. ### Claim 5 (depends on 1) The bioenzymatic, concentrated cleaning product of claim 1, wherein the suspending agent comprises xanthan gum. ### Claim 6 (depends on 1) The bioenzymatic, concentrated cleaning product of claim 1, wherein the suspending and/or rheology modifying agent is present at about 0.1-2% by weight. ### Claim 7 (depends on 1) The bioenzymatic, concentrated cleaning product of Claim 1, wherein the odor control agent is selected from the group consisting of fragrances, malodor control agents, and combinations thereof. ### Claim 8 (depends on 1) The bioenzymatic, concentrated cleaning product of claim 1, wherein the odor control agent is present at about 0.1-2% by weight. ### Claim 9 (depends on 1) The bioenzymatic, concentrated cleaning product of claim 1, wherein the pH adjuster is selected from the group consisting of organic acids, mineral acids, and combinations thereof. ### Claim 10 (depends on 1) The bioenzymatic, concentrated cleaning product of claim 1, wherein the pH adjuster comprises lactic acid. ### Claim 11 (depends on 1) The bioenzymatic, concentrated cleaning product of claim 1, wherein the water is present in an amount of at least about 45% by weight. ### Claim 12 A bioenzymatic, concentrated cleaning product, comprising: 15-55% by weight bacillus spores having an average particle diameter of about 5-50 microns; about 0.1-2% by weight of a suspending and/or rheology modifying agent; about 0.1-2% by weight of an odor control agent; an acidic pH adjuster; and a balance consisting essentially of water; wherein the 15-55% by weight bacillus spores are able to remain suspended in the water for two years under static conditions and the bioenzymatic, concentrated cleaning product is substantially free of organic solvents. ### Claim 13 (depends on 12) The bioenzymatic. concentrated cleaning product of claim 12, wherein the suspending agent comprises a gum. ### Claim 14 (depends on 12) The bioenzymatic, concentrated cleaning product of claim 12, wherein the odor control agent produces a citrus odor. ### Claim 15 (depends on 12) The bioenzymatic, concentrated cleaning product of claim 12, wherein the pH adjuster comprises sufficient acid to maintain a pH of about 5.0-9.5. ### Claim 16 (depends on 12) The bioenzymatic, concentrated cleaning product of claim 12, wherein the pH adjuster comprises sufficient acid to maintain a pH of about 6.0-8.0. ### Claim 17 (depends on 12) The bioenzymatic, concentrated cleaning product of claim 12, wherein the bacillus spores arc present at 15-35% by weight. ### Claim 18 (depends on 12) The bioenzymatic, concentrated cleaning product of claim 12, wherein the water is present in an amount of at least about 45% by weight. ### Claim 19 A method of preparing a bioenzymatic cleaning product from a concentrate, comprising the steps of: mixing 15-55% by weight bacillus spores with a suspending and/or rheology modifying agent, an odor control agent, sufficient pH adjuster to maintain a pH of about 5.0-9.5, and a balance consisting essentially of water, to form a concentrate; the 15-55% by weight bacillus spores in the concentrate having an average diameter of 5-50 microns and remaining suspended in the water under static conditions; and diluting the concentrate with water using a proportioner system, to yield the bioenzymatic cleaning product; wherein the bioenzymatic cleaning product is substantially free of organic solvents.