# US 10,246,493 B2 · De novo structural protein design for manufacturing high strength materials

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- Number: US 10,246,493 B2
- Document ID: US10246493B2
- Status: Granted
- Kind: B2
- Application number: 16/043,935
- Filed: 2018-07-24
- Published: 2019-01-24
- Granted: 2019-04-02
- Earliest claimed: 2015-04-24
- Assignees: The Penn State Research Foundation
- Applicants: The Penn State Research Foundation
- Inventors: Melik Demirel; Benjamin Allen; Huihun Jung
- Web page: https://patentgrep.com/patent/US10246493B2

## CPC

- C07K 14/00: Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K 14/43504: {from invertebrates}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Provided is a supramolecular polypeptide comprising alternating repeats of crystallite-forming subsequences and amorphous subsequences. The crystallite-forming subsequences form crystallites comprising stacks of one or more β-sheets and the amorphous subsequences form a network of hydrogen bonds. The supramolecular polypeptides are capable of exhibiting self-healing behavior.

## Claims

### Claim 1

A method of making a synthetic or recombinant polypeptide capable of forming at least a first crystal-forming domain block and at least a first amorphous domain block, the method comprising selecting an amino acid sequence that is capable of forming the first crystal domain block, wherein the crystal domain block is from about 2 nm to about 5 nm long and comprises from 10 to 30 amino acids, and selecting an amino acid sequence that is capable of forming a first amorphous domain block, wherein the first amorphous domain block is about 3 nm long and comprises about 15 amino acids, and forming the synthetic or recombinant polypeptide by incorporating [i] the amino acid sequence comprising AA[XXXXXX]HH (SEQ ID NO: 313), wherein the XXXXXX comprises histidine, threonine, valine, alanine, serine, or a combination thereof that is capable of forming the first crystal-forming domain block and [ii] the amino acid sequence comprising a glycine rich sequence according to Table 6 that is capable of forming the first amorphous domain block into the synthetic or recombinant polypeptide.