# US 10,246,490 B2 · Conotoxin peptide κ-CPTX-BTL02, preparation method therefor, and uses thereof

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,246,490 B2
- Document ID: US10246490B2
- Status: Granted
- Kind: B2
- Application number: 15/539,720
- Filed: 2014-12-26
- Published: 2018-06-14
- Granted: 2019-04-02
- Earliest claimed: 2014-12-26
- Assignees: Bgi Shenzhen
- Applicants: Bgi Shenzhen
- Inventors: Jie Liu; Zhilong Lin; Bo Wen; Ting Tong; Fen Mo; Chao Peng; Qiong Shi
- Web page: https://patentgrep.com/patent/US10246490B2

## CPC

- C07K 7/08: having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68)
- A61K 38/10: Peptides having 12 to 20 amino acids {(A61K38/043 - A61K38/046 take precedence)}
- C07K 1/00: General methods for the preparation of peptides {, i.e. processes for the organic chemical preparation of peptides or proteins of any length}
- C07K 1/061: {using protecting groups}
- C12N 5/10: Cells modified by introduction of foreign genetic material
- C12N 15/63: Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Provided is a new conotoxin peptide κ-CPTx-btl02. The conotoxin peptide κ-CPTx-btl02 has derivative polypeptide that has an amino acid sequence indicated by SEQ ID NO:1, or has derivative polypeptide that has an amino acid sequence indicated by SEQ ID NO:1 and that is formed after substitution, addition or deletion of one or more amino acids are performed and has original functions. Also provided is polynucleotide for coding the peptide, comprising a constructed body of the polynucleotide, an expression carrier and transformed cells. A peptide preparation method and uses in the treatment of diseases related to a calcium ion channel.

## Claims

### Claim 1

A method of inhibiting a calcium ion channel, the method comprising contacting the calcium ion channel with a conotoxin peptide κ-CPTx-btl02, wherein the peptide comprises the amino acid sequence of SEQ ID NO: 1, and wherein the peptide contains three pairs of disulfide bonds.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the calcium ion channel is a high-voltage activated calcium ion channel.

### Claim 3

A method of inhibiting a calcium ion channel in a subject in need thereof, the method comprising administering to the subject a conotoxin peptide κ-CPTx-btl02, wherein the peptide comprises the amino acid sequence of SEQ ID NO: 1, and wherein the peptide contains three pairs of disulfide bonds.

### Claim 4 (depends on 3)

The method according to claim 3, wherein the calcium ion channel is a high-voltage activated calcium ion channel.