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US 10,245,349 B2Granted
Birth tissue material and method of preparation
AssigneeBioDlogics, LLC
InventorTimothy R. Brahm
Life of this patent
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Abstract
A method of treating a soft tissue defect in or on the body of a patient is provided. A placental construct for treatment of a disease or condition is also provided. The placental construct includes a therapeutically effective amount of a birth tissue material.
Claims8
A method of treating a soft tissue defect in or on the body of a patient comprising the steps of:
- providing a placental construct comprising:
- i) a therapeutically effective amount of a human birth tissue material that is cryofrozen, morselized and subsequently suspended in a tissue suspension solution;
- and ii) an amniotic fluid composition;
- and
- administering the placental construct directly into or onto the soft tissue defect,
- wherein the tissue suspension solution comprises about 36% volume of human albumin 25% solution and about 20% volume of dimethyl sulfoxide.
3 more elements
A method of treating a soft tissue defect in or on the body of a patient comprising the step of
- administering a placental construct directly into or onto the soft tissue defect, wherein the placental construct comprises a therapeutically effective amount of a human birth tissue material comprising a tissue suspension and an amniotic fluid composition, the human birth tissue material prepared according to a process comprising the steps of:
- (a) recovering placental tissue components and amniotic fluid from a single seronegative, healthy human via cesarean section or vaginal delivery;
- (b) subjecting the placental tissue components to cryopreservation;
- (c) morselizing the cryopreserved placental tissue components;
- (d) homogenizing the morselized placental tissue components in a tissue suspension solution to form a tissue suspension;
- (e) centrifuging the amniotic fluid from step (a) to form a pellet that is retained and a supernatant that is aspired off;
- (f) suspending the pellet in a cell suspension solution to form an amniotic fluid composition;
- (g) homogenizing the tissue suspension from step (d) with the amniotic fluid composition of step (f) to form a bulk tissue product;
- and
- (h) cryofreezing the bulk tissue product to form the human birth tissue material;
- wherein the tissue suspension solution comprises about 36% volume of human albumin 25% solution and about 20% volume of dimethyl sulfoxide.
8 more elements
Dependencies are inferred from the claim text.