# US 10,245,299 B2 · Autoassembling peptides for the treatment of pulmonary bulla > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,245,299 B2 - Document ID: US10245299B2 - Status: Granted - Kind: B2 - Application number: 15/124,636 - Filed: 2015-03-10 - Published: 2017-03-16 - Granted: 2019-04-02 - Earliest claimed: 2014-03-10 - Assignees: 3-D Matrix, Ltd. - Applicants: 3-D Matrix, Ltd. - Inventors: Manav Mehta; Hisashi Tsukada; Eun Seok Gil; Karl Gilbert; Satoru Kobayashi - Web page: https://patentgrep.com/patent/US10245299B2 ## CPC - A61K 38/16: Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof {(enzyme inhibitors A61K38/005)} - A61B 17/34: Trocars; Puncturing needles - A61K 38/08: Peptides having 5 to 11 amino acids {(A61K38/043 - A61K38/046 take precedence)} - A61K 38/10: Peptides having 12 to 20 amino acids {(A61K38/043 - A61K38/046 take precedence)} - A61M 16/0463: {combined with suction tubes, catheters or the like; Outside connections} - A61P 11/00: Drugs for disorders of the respiratory system - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)} - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS ## Abstract Materials and methods for treatment of pulmonary bulla are provided. A peptide comprising between about 7 amino acids and about 32 amino acids in a solution may be introduced to a target site. A hydrogel barrier may be provided at the target site in order to treat the pulmonary bulla. ## Claims ### Claim 1 A method of treating a pulmonary bulla in a subject, comprising: introducing a delivery device to a target area of the pulmonary bulla of the subject; positioning an end of the delivery device in the target area in which a treatment of the pulmonary bulla is desired; administering, through the delivery device, a solution comprising an amphiphilic self-assembling peptide comprising between about 7 amino acids and 32 amino acids in an effective amount and in an effective concentration to the target area to form a hydrogel barrier under physiological conditions of the target area to treat the pulmonary bulla; removing the delivery device from the target area; and collapsing the pulmonary bulla prior or subsequent to administering the solution. ### Claim 2 (depends on 1) The method of claim 1, wherein a cavity of the pulmonary bulla is filled with the solution through connecting bronchioles. ### Claim 3 (depends on 2) The method of claim 2, further comprising inserting an endotracheal tube through a primary bronchus of a lung of the subject. ### Claim 4 A method of treating a pulmonary bulla in a subject, comprising: introducing a delivery device to a target area of the pulmonary bulla of the subject; positioning an end of the delivery device in the target area in which a treatment of the pulmonary bulla is desired; administering, through the delivery device, a solution comprising an amphiphilic self-assembling peptide comprising between about 7 amino acids and 32 amino acids in an effective amount and in an effective concentration to the target area to form a hydrogel barrier under physiological conditions of the target area to treat the pulmonary bulla, wherein the administering the solution comprises administering the solution in a single dose; administering the solution until a volume of the target area is filled; and removing the delivery device from the target area. ### Claim 5 A method of treating a pulmonary bulla in a subject, comprising: introducing a delivery device to a target area of the pulmonary bulla of the subject; positioning an end of the delivery device in the target area in which a treatment of the pulmonary bulla is desired; administering, through the delivery device, a solution comprising an amphiphilic self-assembling peptide comprising between about 7 amino acids and 32 amino acids in an effective amount and in an effective concentration to the target area to form a hydrogel barrier under physiological conditions of the target area to treat the pulmonary bulla; and removing the delivery device from the target area, wherein the subject has been diagnosed with at least one of emphysema and chronic obstructive pulmonary disease (COPD) prior to administering the solution comprising the amphiphilic self-assembling peptide. ### Claim 6 A method of treating a pulmonary bulla in a subject, comprising: introducing a delivery device to a target area of the pulmonary bulla of the subject; positioning an end of the delivery device in the target area in which a treatment of the pulmonary bulla is desired; administering, through the delivery device, a solution comprising an amphiphilic self-assembling peptide comprising between about 7 amino acids and 32 amino acids in an effective amount and in an effective concentration to the target area to form a hydrogel barrier under physiological conditions of the target area to treat the pulmonary bulla; and removing the delivery device from the target area, wherein the solution comprising the amphiphilic self-assembling peptide comprises (RADA)4 (SEQ ID NO: 1) at a concentration of about 0.5 weight per volume (w/v) percent. ### Claim 7 (depends on 6) The method of claim 6, wherein the solution comprising the amphiphilic self-assembling peptide comprises a concentration of calcium chloride of about 0.125 M. ### Claim 8 A method of treating a pulmonary bulla in a subject, comprising: introducing a delivery device to a target area of the pulmonary bulla of the subject; positioning an end of the delivery device in the target area in which a treatment of the pulmonary bulla is desired; administering, through the delivery device, a solution comprising an amphiphilic self-assembling peptide comprising between about 7 amino acids and 32 amino acids in an effective amount and in an effective concentration to the target area to form a hydrogel barrier under physiological conditions of the target area to treat the pulmonary bulla; removing the delivery device from the target area; and preparing the solution comprising the amphiphilic self-assembling peptide by adding the amphiphilic self-assembling peptide to a buffer comprising at least two salts and adding the buffer to the solution, wherein the amphiphilic self-assembling peptide is selected from the group consisting of (RADA)4 (SEQ ID NO: 1), (IEIK)3I (SEQ ID NO: 2), and (KLDL)3 (SEQ ID NO: 3).