# US 10,245,290 B2 · Probiotic strains for use in treatment or prevention of osteoporosis

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- Number: US 10,245,290 B2
- Document ID: US10245290B2
- Status: Granted
- Kind: B2
- Application number: 14/782,138
- Filed: 2014-04-03
- Published: 2016-03-10
- Granted: 2019-04-02
- Earliest claimed: 2013-04-03
- Assignees: Probi AB
- Applicants: Probi AB
- Inventors: Anna Berggren; Niklas Larsson; Gunilla Önning; Irini Lazou Ahrén; Klara Sjögren; Claes Ohlsson
- Web page: https://patentgrep.com/patent/US10245290B2

## CPC

- A61K 35/747: Lactobacilli, e.g. L. acidophilus or L. brevis
- A23L 33/135: Bacteria or derivatives thereof, e.g. probiotics
- A61P 19/10: for osteoporosis
- C12N 1/205: Bacterial isolates
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- C12R 2001/225: Lactobacillus
- C12R 2001/25: Lactobacillus plantarum
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present invention relates to at least one probiotic strain chosen from Lactobacillus paracasei, or at least one probiotic strain chosen from Lactobacillus paracaseiin combination with at least one probiotic strain chosen from Lactobacillus plantarum, for use in the treatment or prevention of osteoporosis or for use in increasing the absorption of at Ca2+ ions, in a mammal, preferably in a human.

## Claims

### Claim 1

A method for treating or inhibiting osteoporosis, treating or inhibiting bone loss, or increasing the absorption of Ca2+ ions, comprising orally administering to a mammal a composition comprising a therapeutically effective amount of at least one probiotic strain selected from the group consisting of Lactobacillus paracasei 8700:2, DSM 13434, and Lactobacillus paracasei 02:A, DSM 13432, wherein the composition is supplemented with additional Ca2+ in the form of a salt, wherein the inhibition of osteoporosis or bone loss is by reducing osteoporosis or bone loss compared to osteoporosis or bone loss without administration of said composition, and wherein increasing the absorption of Ca2+ ions is compared to absorption of Ca2+ ions without administration of said composition.

### Claim 2 (depends on 1)

The method of claim 1, wherein said mammal is a human.

### Claim 3 (depends on 1)

The method of claim 1, wherein the treatment or inhibition of osteoporosis comprises inhibiting one or more of cortical bone loss, bone mineral content loss, and bone-resorption.

### Claim 4 (depends on 1)

The method of claim 1, wherein said at least one probiotic strain of Lactobacillus paracasei is provided in combination with at least one probiotic strain of Lactobacillus plantarum.

### Claim 5 (depends on 4)

The method of claim 4, wherein said at least one probiotic strain of Lactobacillus plantarum is one or more selected from the group consisting of Lactobacillus plantarum 299, DSM 6595, Lactobacillus plantarum 299v, DSM 9843, Lactobacillus plantarum HEAL 9, DSM 15312, Lactobacillus plantarum HEAL 19, DSM 15313, and Lactobacillus plantarum HEAL 99, DSM 15316.

### Claim 6 (depends on 1)

The method of claim 1, wherein said at least one probiotic strain(s) is present in a composition comprising at least one carrier.

### Claim 7 (depends on 6)

The method of claim 6, wherein said carrier is selected from the group consisting of a food product, a dietary supplement, a medical food, a functional food, and a nutritional product.

### Claim 8 (depends on 1)

The method of claim 1, wherein at least one of said probiotic strain(s) is present in an amount from about 1×106 to about 1×1014 CFU.

### Claim 9 (depends on 8)

The method of claim 8, wherein at least one of said probiotic strain(s) is present in an amount from about 1×108 to 1×1012 CFU.

### Claim 10 (depends on 8)

The method of claim 8, wherein at least one of said probiotic strain(s) is present in an amount from about 1×109 to 1×1011 CFU.

### Claim 11 (depends on 1)

The method of claim 1, wherein the method is for treating or inhibiting osteoporosis, treating or inhibiting bone loss, or increasing the absorption of Ca2+ ions, in a woman.

### Claim 12 (depends on 5)

The method of claim 5, wherein said at least one probiotic strain comprises Lactobacillus paracasei 8700:2, DSM 13434, in combination with Lactobacillus plantarum HEAL 9, DSM 15312, and Lactobacillus plantarum HEAL 19, DSM 15313.

### Claim 13 (depends on 11)

The method of claim 11, wherein the method is for treating or inhibiting osteoporosis, treating or inhibiting bone loss, or increasing the absorption of Ca2+ ions, in a premenopausal or postmenopausal woman.

### Claim 14 (depends on 1)

The method of claim 1, wherein the salt is selected from the group consisting of calcium carbonate, calcium chloride, calcium salts of citric acid, calcium gluconate, calcium glycerophosphate, calcium lactate, calcium oxide, and calcium sulphate.

### Claim 15

A method for treating or inhibiting osteoporosis, treating or inhibiting bone loss, or increasing the absorption of Ca2+ ions, in a woman, comprising orally administering to a woman a therapeutically effective amount of at least one probiotic strain selected from the group consisting of Lactobacillus paracasei 8700:2, DSM 13434, and Lactobacillus paracasei 02:A, DSM 13432, wherein the inhibition of osteoporosis or bone loss is by reducing osteoporosis or bone loss compared to osteoporosis or bone loss without administration of said at least one probiotic strain, and wherein increasing the absorption of Ca2+ ions is compared to absorption of Ca2+ ions without administration of said composition.

### Claim 16 (depends on 15)

The method of claim 15, wherein the woman is a premenopausal or postmenopausal woman.

### Claim 17 (depends on 15)

The method of claim 15, wherein the treatment or inhibition of osteoporosis comprises inhibiting one or more of cortical bone loss, bone mineral content loss, and bone-resorption.

### Claim 18 (depends on 15)

The method of claim 15, wherein said at least one probiotic strain of Lactobacillus paracasei is provided in combination with at least one probiotic strain of Lactobacillus plantarum.

### Claim 19 (depends on 18)

The method of claim 18, wherein said at least one probiotic strain of Lactobacillus plantarum is one or more selected from the group consisting of Lactobacillus plantarum 299, DSM 6595, Lactobacillus plantarum 299v, DSM 9843, Lactobacillus plantarum HEAL 9, DSM 15312, Lactobacillus plantarum HEAL 19, DSM 15313, and Lactobacillus plantarum HEAL 99, DSM 15316.

### Claim 20 (depends on 19)

The method of claim 19, wherein said at least one probiotic strain comprises Lactobacillus paracasei 8700:2, DSM 13434, in combination with Lactobacillus plantarum HEAL 9, DSM 15312 and Lactobacillus plantarum HEAL 19, DSM 15313.

### Claim 21 (depends on 15)

The method of claim 15, wherein said at least one probiotic strain(s) is present in a composition comprising at least one carrier.

### Claim 22 (depends on 21)

The method of claim 21, wherein said carrier is selected from the group consisting of a food product, a dietary supplement, a medical food, a functional food, and a nutritional product.

### Claim 23 (depends on 15)

The method of claim 15, wherein at least one of said probiotic strain(s) is present in an amount from about 1×106 to about 1×1014 CFU.

### Claim 24 (depends on 23)

The method of claim 23, wherein at least one of said probiotic strain(s) is present in an amount from about 1×108 to about 1×1012 CFU.

### Claim 25 (depends on 24)

The method of claim 24, wherein at least one of said probiotic strain(s) is present in an amount from about 1×109 to about 1×1011 CFU.

### Claim 26 (depends on 15)

The method of claim 15, wherein said composition is supplemented with additional Ca2+ in the form of a salt.

### Claim 27 (depends on 26)

The method of claim 26, wherein the salt is selected from the group consisting of calcium carbonate, calcium chloride, calcium salts of citric acid, calcium gluconate, calcium glycerophosphate, calcium lactate, calcium oxide, and calcium sulphate.

### Claim 28 (depends on 2)

The method of claim 2, wherein the human is healthy.

### Claim 29 (depends on 11)

The method of claim 11, wherein the woman is healthy.

### Claim 30 (depends on 15)

The method of claim 15, wherein the woman is healthy.