# US 10,245,279 B2 · Use of immobilization particles for removal of microorganisms and/or chemicals

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- Number: US 10,245,279 B2
- Document ID: US10245279B2
- Status: Granted
- Kind: B2
- Application number: 15/002,300
- Filed: 2016-01-20
- Published: 2016-05-19
- Granted: 2019-04-02
- Earliest claimed: 2009-02-26
- Assignees: NuBiome, Inc.
- Applicants: NuBiome, Inc.
- Inventors: Brian C. Lue
- Web page: https://patentgrep.com/patent/US10245279B2

## CPC

- A61K 31/713: Double-stranded nucleic acids or oligonucleotides
- A61K 9/0031: {Rectum, anus}
- A61K 9/0053: {Mouth and digestive tract, i.e. intraoral and peroral administration}
- A61K 9/02: Suppositories; Bougies; Bases therefor; {Ovules}(apparatus for making A61J3/08; devices for introducing into the body A61M31/00)
- A61K 9/06: Ointments; Bases therefor; {Other semi-solid forms, e.g. creams, sticks, gels (composition of ointments, creams or gels A61K47/00)}
- A61K 9/4841: {Filling excipients; Inactive ingredients}
- A61K 9/7007: {Drug-containing films, membranes or sheets (A61K9/0041, A61K9/0043, A61K9/006, A61K9/0063 take precedence)}
- C07K 16/08: against material from viruses
- C07K 16/12: against material from bacteria
- C07K 16/14: against material from fungi, algea or lichens
- C12Q 1/24: Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
- G01N 33/569: for microorganisms, e.g. protozoa, bacteria, viruses
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Method for immobilizing a target microorganism or target chemical found in a mammal includes introducing into a gastrointestinal tract of the mammal immobilization particles including immobilization molecules capable of attaching to the target microorganism or the target chemical, which immobilization molecules are attached to one or more portions of a structure that is capable of inhibiting contact between tissues of the gastrointestinal tract and the target microorganisms or target chemicals attached to the immobilization molecules.

## Claims

### Claim 1

A method for immobilizing a target microorganism or target chemical found in a gastrointestinal tract of a mammal, the method comprising:
introducing into a gastrointestinal tract of the mammal an immobilization particle comprising a flat substrate, one or more further structures disposed on one side of the flat substrate, and immobilization molecules attached to one or more portions of the one side of the flat substrate and not to the one or more further structures, which immobilization molecules being capable of attaching to the target microorganism or the target chemical; and
wherein the one or more further structures comprise columns having a height in a range from about 0.01 microns to about 500 microns and being spaced apart in a range from about 0.1 microns to about 500 microns.

### Claim 2 (depends on 1)

The method of claim 1 further comprising removing the immobilization particles from the gastrointestinal tract.

### Claim 3 (depends on 2)

The method of claim 2 wherein removing includes removing by administering an enema.

### Claim 4 (depends on 2)

The method of claim 2 wherein removing the target microorganism entails removing the target microorganism without breaking the target microorganism into pieces.

### Claim 5 (depends on 1)

The method of claim 1 wherein the one or more further structures inhibit contact between tissues of the gastrointestinal tract and/or dendritic cells attached to a wall of the gastrointestinal tract and the target microorganisms or target chemicals attached to the immobilization molecules.

### Claim 6 (depends on 1)

The method of claim 1 wherein the immobilization particles are introduced into the gastrointestinal tract orally or via an anus of the mammal.

### Claim 7 (depends on 6)

The method of claim 6 wherein the immobilization particles are introduced via the anus using a suppository comprised of a capsule containing the immobilization particles or an enema wherein the immobilization particles are carried in a liquid.

### Claim 8 (depends on 6)

The method of claim 6 wherein the immobilization particles are introduced orally using a capsule, a tablet or a liquid.

### Claim 9 (depends on 6)

The method of claim 6 wherein immobilization particles are orally delivered in a liquid mixture inside a sealed capsule.

### Claim 10 (depends on 9)

The method of claim 9 wherein the liquid comprises a preservative and a food-based oil or a water-based saline solution.

### Claim 11 (depends on 6)

The method of claim 6 wherein the immobilization particles are disposed inside a gel-cap which is coated with a substance that dissolves when the pH of the environment reaches about 7, whereby the immobilization particles are delivered orally into a large intestine without being deployed above the large intestine.

### Claim 12 (depends on 11)

The method of claim 11 wherein the substance includes pharmaceutical grade shellac or a methylacrylic acid/methyl methacrylate copolymer.

### Claim 13 (depends on 6)

The method of claim 6 wherein the immobilization particles are disposed inside a gel-cap that survives stomach acid and dissolves upon entry into the small intestine, whereby the immobilization particles are delivered orally into a small intestine.

### Claim 14 (depends on 6)

The method of claim 6 wherein the immobilization particles are disposed inside a gel-cap that is coated with a substance that survives stomach acid and dissolves upon reaching a predetermined location in the small intestine.

### Claim 15 (depends on 1)

The method of claim 1 wherein the immobilization molecules are one or more of antibodies or aptamers.

### Claim 16 (depends on 15)

The method of claim 15 wherein the aptamer is one or more of a single-stranded RNA, a DNA molecule or a peptide.

### Claim 17 (depends on 15)

The method of claim 15 wherein the flat substrate is a gold substrate.

### Claim 18 (depends on 15)

The method of claim 15 wherein the flat substrate is a gold substrate disposed on a polymer substrate support.

### Claim 19 (depends on 15)

The method of claim 15 wherein the flat substrate is a polymer substrate.