Method of forming an imaging calibration device
AssigneeFFEI Limited
InventorsMartin Philip Gouch, Craig Revie, Peter Walsh, Jacqueline Margaret Deane
Life of this patent
Estimating the term…
Abstract
A method of forming an imaging calibration device for a biological material imaging system, the method comprises: providing one or more discrete regions upon or within a retaining member, each region for the receipt of a selected predetermined biological stain material; selecting one or more predetermined biological stain materials, wherein each of the selected predetermined biological stain materials has a predetermined optical response, and providing one or more of the selected predetermined biological stain materials to the said one or more discrete regions such that the said material is localised in the said region.
Claims27
A method of forming an imaging calibration device for a bright field biological material imaging system, the method comprising:
- providing one or more discrete regions of a uniform tissue substitute upon or within a retaining member, each region for the receipt of a selected non-fluorescent tissue stain material, wherein, for each discrete region, every part of the discrete region produces a common optical path length through the region, as defined in the direction normal to the surface of the retaining member;
- selecting one or more non-fluorescent tissue stain materials, wherein each of the selected non-fluorescent tissue stain materials has a predetermined optical response;
- and
- depositing one or more of the selected non-fluorescent tissue stain materials in a fluid form to the said one or more discrete regions such that the said material is localised in the said region and each said discrete region produces a homogenous spectral response across the region.
An imaging calibration device for a bright field biological material imaging system, the device comprising:
- a retaining member;
- one or more discrete regions of a uniform tissue substitute located upon or within the retaining member, each region for the receipt of a selected non-fluorescent tissue stain material, wherein every part of each discrete region has a common optical path length through the region, as defined in the direction normal to the surface of the retaining member;
- and
- one or more selected non-fluorescent tissue stain materials received in the said one or more discrete regions such that the said material is localised in the said region, wherein each of the selected non-fluorescent tissue stain materials has a predetermined optical response, and wherein each discrete region produces a homogenous spectral response across the region.
A device to calibrate a bright field biological material imaging system, the device comprising:
- one or more discrete regions of a uniform tissue substitute located upon or within the retaining member, each region for the receipt of a non-fluorescent tissue stain material, wherein every part of each discrete region has a common optical path length through the region, as defined in the direction normal to the surface of the retaining member;
- and
- one or more non-fluorescent tissue stain materials received in the said one or more discrete regions such that the said material is localised in the said region,
- wherein each of the one or more discrete regions produces a homogenous spectral response across the region.
Dependencies are inferred from the claim text.