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US 10,241,119 B2Granted
High sensitivity measurement of parathyroid hormone-related peptide using LC-MS/MS and associated methods
AssigneeUniversity of Utah Research Foundation
InventorsMark M. Kushnir, Alan L. Rockwood, A. Wayne Meikle
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Abstract
Methods for measuring and analyzing parathyroid hormone-related peptide (PTHrP) using LC-MS/MS, including applications of the methods thereof, are disclosed and discussed. Such methods can include, along with the use of an isotope-labeled internal standard, purifying PTHrP from a biological sample, proteolytically digesting the PTHrP, and measuring specific digestion products using the using LC-MS/MS.
Claims16
A method of measuring parathyroid hormone-related peptide (PTHrP) in a biological sample, comprising:
- preparing the biological sample, wherein preparing further comprises:
- extracting PTHrP peptides from the biological sample;
- mixing a PTHrP sequence-derived isotope-labeled internal standard (IS) with the PTHrP peptides;
- proteolytically digesting the PTHrP peptides and the IS to produce a digestion product;
- and
- chromatographically separating the digestion product;
- and
- selecting and subjecting a chromatographic peak of the separated digestion product to tandem mass spectrometry to determine the amount of a target peptide, wherein the sequence of the target peptide is YLTQETNK (SEQ ID NO:
- 002) and the sequence of the IS is YLTQETNK (SEQ ID NO:
- 002).
7 more elements
Dependencies are inferred from the claim text.