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AssigneeWaters Technologies Corporation
InventorsErin E. Chambers, Martha Stapels, Joanne Mather
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A method for identifying a polypeptide a specimen can include (i) treating a specimen suspected of including an insulin with a base; (ii) extracting a first fraction of the treated specimen by solid phase extraction using a mixed mode or polymeric reversed-phase media and a first solvent including an acid; (iii) separating a component of the first fraction by liquid chromatography using a chromatographic surface including a hydrophobic surface group and one or more ionizable modifiers, and a second solvent including an acid; and (iv) analyzing the component of the first fraction by mass spectroscopy, thereby identifying the polypeptide, if present, using a signal corresponding to a sequence fragment ion from the polypeptide. The signal can correspond to an intact multiply charged precursor fragment selected in a first quadrupole and its corresponding sequence fragment ion selected in a final quadrupole.
A method for identifying a polypeptide in a specimen, the method comprising:
A method for assessing the bioequivalence of a first polypeptide and a second polypeptide, the method comprising:
Dependencies are inferred from the claim text.