Plasmonic biosensors with built-in artificial antibodies
AssigneeWashington University in St. Louis
InventorsSrikanth Singamaneni, Limei Tian, Keng-Ku Liu, Abdennour Abbas, Jeremiah J. Morrissey, Evan D. Kharasch
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Abstract
Plasmonic nanotransducers, methods of preparing plasmonic nanotransducers, and methods for label-free detection of target molecules are disclosed. The plasmonic nanotransducers include hollow nanostructure cores and artificial antibodies. The plasmonic nanotransducers are exposed to a biological sample that can contain the specific target molecules. The plasmonic nanotransducers can be analyzed with surface enhanced Raman scattering techniques and/or localized surface plasmon resonance techniques to quantify the amount of the target molecule in the sample.
Claims20
A plasmonic nanotransducer comprising:
- a hollow nanostructure core, wherein the hollow nanostructure core is selected from the group consisting of a nanocage, a nanorattle, a nanoshell, and a nanomatryoshka;
- and
- functional monomers polymerized to the hollow nanostructure core, wherein the polymerized functional monomers comprise at least one recognition cavity that is substantially complementary to a target molecule.
A method of preparing a plasmonic nanotransducer comprising a hollow nanostructure core and functional monomers adhered to the hollow nanostructure core, the method comprising
- synthesizing a hollow nanostructure core, wherein the hollow nanostructure core is selected from the group consisting of a nanocage, a nanorattle, a nanoshell, and a nanomatryoshka;
- immobilizing at least one template molecule on the surface of the hollow nanostructure core to form a template molecule-nanostructure core structure;
- polymerizing functional monomers onto the template molecule-nanostructure core structure;
- and
- removing the template molecule to form at least one recognition cavity in the polymerized functional monomers upon removal of the template molecule, wherein the at least one recognition cavity is substantially complementary to a target molecule.
A label-free method for detecting a target molecule in a biological sample, the method comprising:
- obtaining a biological sample from a subject;
- contacting the biological sample with a plasmonic nanotransducer, wherein the plasmonic nanotransducer comprises:
- a hollow nanostructure core, wherein the hollow nanostructure core is selected from the group consisting of a nanocage, a nanorattle, a nanoshell, and a nanomatryoshka;
- and
- functional monomers polymerized to the hollow nanostructure core, wherein the polymerized functional monomers comprise at least one recognition cavity that is substantially complementary to a target molecule;
- wherein the target molecule in the biological sample forms a complex with the plasmonic nanotransducer;
- and
- detecting the complex.
Dependencies are inferred from the claim text.