Bacterial detection platform based on SERS mapping
AssigneeUniversity of Massachusetts
InventorLili He
Life of this patent
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Abstract
A bacterial detection platform integrating the sensitive SERS technique and the advanced mapping technique. Bacterial cells on the SERS substrate are detected using the mapping technique. The identification is based on the fingerprint of the bacterial SERS spectra. The quantification of the cells is based on the mapping technique. For different applications, silver or gold nanoparticles can be integrated onto a filter membrane for concentration and detection of bacterial cells in water or silver dendrites can be used as the SERS substrate. The SERS substrates are also modified with capturers and fixed in a vessel to concentrate cells from complex liquid matrices.
Claims17
A method, based on label-free surface-enhanced Raman scattering (SERS) mapping, for detecting bacterial cells in bacterial mixtures, the method comprising:
- capturing bacterial cells on a SERS substrate comprising nanostructures that are silver nanoparticles, gold nanoparticles, silver dendrites, or mixtures thereof, the nanoparticles located on a solid support, wherein individual bacterial cells are bound by individual bacterial capturers that are conjugated to individual nanoparticles and comprise a boronic acid;
- collecting from the SERS substrate with the captured bacterial cells, using a Raman microscope, a number of spectra at each pixel in a defined area of a pixelated detector;
- generating a false color map of predetermined spectral regions;
- and
- detecting the captured bacterial cells from the false color map.
Dependencies are inferred from the claim text.