Systems and methods for camera-based image processing in microscopy instruments
AssigneeGE Healthcare Bio-Sciences AB
InventorPaul C. Goodwin
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Abstract
Systems and methods for executing super-resolution microscopy of a specimen with most of the image processing performed in a camera of a fluorescence microscopy instrument are described. In one aspect, the camera includes one or more processors to execute machine-readable instructions that control excitation light output from a multi-channel light source, control capture of intermediate images of the specimen, and perform image processing of the intermediate images to produce a final super-resolution image of the specimen.
Claims20
A camera comprising:
- one or more processors;
- and
- a computer-readable medium having machine-readable instructions recorded thereon,
- wherein the one or more processors is configured to execute the machine-readable instructions for:
- capturing light emitted from a subset of light emitters within a specimen exposed to a light source, to record an unprocessed intermediate image of the subset of light emitters in the camera;
- processing the recorded unprocessed intermediate image and creating a processed intermediate image of the subset of light emitters, wherein the processed intermediate image comprises a plurality of spots, each spot associated with an individual image of a light emitter of the subset of light emitters;
- and
- obtaining, for each individual image, a centroid coordinate of said each individual image.
A method for capturing and processing images of a specimen using a camera, the method comprising:
- capturing light emitted from a subset of light emitters within the specimen being exposed to a light source, to record an unprocessed intermediate image of the subset of light emitters in the camera;
- using the camera to process the recorded unprocessed intermediate image and create a processed intermediate image of the subset of light emitters, wherein the processed intermediate image comprises a plurality of spots, each spot associated with an individual image of a light emitter of the subset of light emitters;
- using the camera to obtain, for each individual image, a centroid coordinate of said each individual image.
Dependencies are inferred from the claim text.