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US 10,241,023 B2Granted
Sample analysis for mass cytometry
AssigneeFluidigm Canada Inc.
InventorAlexandre Loboda
Life of this patent
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Abstract
The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
Claims18
A laser ablation inductively coupled plasma mass spectrometry system comprising:
- a laser ablation source for generating an ablated plume from a sample;
- a laser for emitting a laser beam;
- a mirror positioned to reflect the laser beam onto the sample, wherein the mirror includes an opening;
- and
- an injector gas conduit adapted to couple the laser ablation source with an inductively coupled plasma (ICP) produced by an ICP torch, wherein said injector gas conduit passes through the opening of the mirror, wherein the injector gas conduit includes an injector inlet positioned within the laser ablation source, and wherein the injector inlet is configured for capturing the ablated plume.
2 more elements
A method of using a laser ablation inductively coupled plasma mass spectrometry system, the method comprising:
- directing pulses of a laser beam to a plurality of sites of a sample for generating a set of ablated plumes of the sample, wherein directing a pulse of the laser beam comprises reflecting the pulse of the laser beam onto the sample to generate an ablated plume using a mirror having an opening;
- capturing each ablated plume of the set of ablated plumes;
- transferring each of the captured ablated plumes into an inductively coupled plasma (ICP), wherein transferring each of the captured ablated plumes comprises passing each of the captured ablated plumes through the opening of the mirror;
- and
- ionizing each of the captured and transferred ablated plumes in the ICP;
- wherein the system comprises:
- a laser ablation source for generating the set of ablated plumes from the sample;
- a laser for emitting the laser beam;
- the mirror positioned to reflect the laser beam onto the sample;
- and
- an injector gas conduit adapted to couple the laser ablation source with the ICP produced by an ICP torch, wherein said injector gas conduit passes through the opening of the mirror, wherein the injector includes an injector inlet positioned within the laser ablation source, and wherein the injector inlet is configured for capturing the set of ablated plumes.
8 more elements
Dependencies are inferred from the claim text.