Method for diluted plasma separation using container for blood dilution and storage containing gelling agent for plasma separation

A blood analysis structure for accommodating a bioanalytical sample prepared by diluting a minute amount of collected blood, comprising:

• a dilution buffer containing an internal standard for diluting plasma in the collected blood;
• a polymer gel for plasma separation for separating the diluted plasma from blood cells in the collected blood;
• a container for blood dilution and storage for accommodating the dilution buffer and the polymer gel for plasma separation and receiving and accommodating the collected blood so as to store the collected blood;
• and
• a sealing cap for enclosing the blood to be introduced from the upper opening of the container for blood dilution and storage together with the dilution buffer and the polymer gel for plasma separation into the container for blood dilution and storage,
• wherein the dilution buffer and the polymer gel for plasma separation are allowed to separately form independent layers disposed in the vertical direction within the inner space of the container for blood dilution and storage,
• wherein the collected blood is introduced from the upper opening and a centrifugal force is applied to the container for blood dilution and storage in a downward direction, thereby allowing a plasma layer of the dilution buffer containing plasma of the collected and diluted blood, a layer of the polymer gel for plasma separation, and a layer of the blood cells to be separately formed as independent layers in the container for blood dilution and storage,
• wherein the specific gravity of the dilution buffer is lower than that of the plasma, and
• wherein the specific gravity of the plasma layer of the diluted plasma in the collected blood is approximately 1.012 to 1.014.

A method for diluted blood separation for accommodating a bioanalytical sample prepared by diluting a minute amount of collected blood to separate diluted plasma from blood cells, comprising the steps of:

• preparing a dilution buffer for diluting plasma in the collected blood;
• preparing a polymer gel for plasma separation for separating the diluted plasma from blood cells in the collected blood;
• preparing a container for blood dilution and storage for accommodating the dilution buffer and the polymer gel for plasma separation and receiving and accommodating the collected blood so as to store the collected blood;
• preparing a sealing cap for enclosing the blood to be introduced from the upper opening of the container for blood dilution and storage together with the dilution buffer and the polymer gel for plasma separation into the container for blood dilution and storage;
• introducing the dilution buffer and the polymer gel for plasma separation in a manner such that they separately form independent layers disposed in the vertical direction within the inner space of the container for blood dilution and storage;
• introducing the collected blood from the upper opening;
• and
• applying a centrifugal force to the container for blood dilution and storage in a downward direction, thereby
• allowing a plasma layer of the dilution buffer containing plasma of the collected and diluted blood, a layer of the polymer gel for plasma separation, and a layer of the blood cells to be separately formed as independent layers in the container for blood dilution and storage, wherein
• the specific gravity of the plasma layer of the diluted plasma in the collected blood is approximately 1.012 to 1.014.

A method for diluted blood separation for accommodating a bioanalytical sample prepared by diluting a minute amount of collected blood to separate diluted plasma from blood cells, comprising the steps of:

• preparing a dilution buffer containing an internal standard for diluting plasma in the collected blood;
• preparing a polymer gel for plasma separation for separating the diluted plasma from blood cells in the collected blood;
• preparing a container for blood dilution and storage for accommodating the dilution buffer and the polymer gel for plasma separation and receiving and accommodating the collected blood so as to store the collected blood;
• preparing a sealing cap for enclosing the blood to be introduced from the upper opening of the container for blood dilution and storage together with the dilution buffer and the polymer gel for plasma separation into the container for blood dilution and storage;
• introducing the dilution buffer and the polymer gel for plasma separation in a manner such that they separately form independent layers disposed in the vertical direction within the inner space of the container for blood dilution and storage;
• introducing the collected blood from the upper opening;
• and
• applying a centrifugal force to the container for blood dilution and storage in a downward direction, thereby allowing a plasma layer of the dilution buffer containing plasma of the collected and diluted blood, a layer of the polymer gel for plasma separation, and a layer of the blood cells to be separately formed as independent layers in the container for blood dilution and storage,
• wherein the specific gravity of the plasma layer of the diluted plasma in the collected blood is approximately 1.012 to 1.014.