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US 10,240,211 B2Granted
Systems and methods for pericarp genotyping
AssigneePioneer Hi-Bred International, Inc.
InventorsJustin Schares, Yue Yun, Chengfeng Zhao
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Abstract
The invention includes methods for obtaining samples of maternal tissue from seeds, obtaining genetic material from the maternal seed tissue, and performing a molecular analysis on the genetic material from the maternal seed tissue to determine maternal lineage of a single seed. The invention also includes methods for establishing a consensus maternal genotype from maternal seed tissues obtained from multiple seeds as well as methods for determining paternal lineage.
Claims3
A method for determining the maternal genotype of a single hybrid maize seed comprising:
- (a) collecting pericarp tissue from a sample consisting of the single hybrid maize seed;
- (b) washing the pericarp tissue from the sample consisting of the single hybrid maize seed with an aqueous 1% sodium dodecyl sulfate (SDS) solution;
- (c) dissociating and homogenizing the pericarp tissue from the sample consisting of the single hybrid maize seed using a cell dissociator to obtain a homogenized solution;
- (d) centrifuging the homogenized solution obtained in step (c) to obtain supernatant;
- and
- (e) genotyping DNA contained within the supernatant, wherein a genotype obtained is a single maternal genotype of the pericarp tissue from the sample consisting of the single hybrid maize seed, and wherein the single hybrid maize seed pericarp genotype is obtained without performing a DNA extraction step.
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Dependencies are inferred from the claim text.