Back to results
US 10,240,197 B1Granted
Methods for analyzing nucleic acids from single cells
Assignee10x Genomics, Inc.
InventorsSydney Brenner, Gi Mikawa, Robert Osborne, Andrew Slatter
Life of this patent
Estimating the term…
Abstract
Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.
Claims26
A method of counting nucleic acids in a sample, the method comprising:
- (a) providing a sample comprising a plurality of cells, wherein a cell of the plurality of cells comprises a plurality of sample polynucleotides;
- (b) generating a plurality of tagged polynucleotides from the plurality of sample polynucleotides of said cell and a plurality of oligonucleotide tags, wherein a tagged polynucleotide of the plurality of tagged polynucleotides comprises:
- (i) a sample sequence from a sample polynucleotide of the plurality of sample polynucleotides;
- (ii) a first tag sequence distinguishing said sample polynucleotide from sample polynucleotides from other cells;
- and
- (iii) a second tag sequence distinguishing said sample polynucleotide from other sample polynucleotides from said cell;
- (c) sequencing the tagged polynucleotide to determine the sample sequence, the first tag sequence, and the second tag sequence;
- and
- (d) using the first tag sequence and the second tag sequence to count a number of sample polynucleotides in said plurality of sample polynucleotides of said cell.
6 more elements
Dependencies are inferred from the claim text.