Sequencing device

A method of preparing reagents, the method comprising:

• inserting a cartridge into an instrument, the cartridge including a plurality of flexible reagent enclosures disposed in a cavity of the cartridge and each reagent enclosure exposing a port to an exterior of the cartridge, each reagent enclosure including a reagent container, the reagent container including an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity, and a reagent contained in the internal cavity;
• connecting a plurality of fluid ports of the instrument to the reagent enclosures, a fluid port of the plurality of fluid ports connecting to the port of one of the reagent enclosures;
• connecting a buffer solution container including buffer solution to the instrument;
• applying the buffer solution through the fluid ports of the instrument to at least partially fill the plurality of reagent enclosures;
• and
• cycling a pressure of the cavity by applying gas through a gas port of the cartridge, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening of the reagent container to form a reagent solution.