Bead emulsion nucleic acid amplification
Assignee454 Life Sciences Corporation
InventorsJan Berka, Yi-Ju Chen, John H. Leamon, Steven Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
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Abstract
Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
Claims10
A composition comprising a population of nucleic acid template-carrying beads each comprising a plurality of species of template nucleic acid molecules, wherein each bead comprises more than 1,000,000 amplified copies of the species of template nucleic acid molecules attached thereto, wherein the population of beads are suspended in a microreactor comprising a water-in-oil emulsion, and wherein a plurality of the microreactors includes only one bead.
Dependencies are inferred from the claim text.