Compositions, kits and related methods for the detection and/or monitoring of Salmonella
AssigneeGen-Probe Incorporated
InventorsMichael R. Reshatoff, Edgar O. Ong, James J. Hogan
Life of this patent
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Abstract
Provided are compositions, kits, and methods for the identification of Salmonella. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.
Claims20
A method for detecting Salmonella in a sample, said method comprising a detecting step of detecting the presence or absence of a Salmonella target nucleic acid using a fluorescently labeled molecular torch oligonucleotide, wherein the molecular torch oligonucleotide comprises the nucleotide sequence consisting essentially of SEQ ID NO:
- 66, 67, 68, 69, or 70, and wherein the detecting step is performed below 60° C.
A composition for use in detecting Salmonella in a sample, said composition comprising (i) a molecular torch oligonucleotide comprising the nucleotide sequence consisting essentially of SEQ ID NO:
- 66, 67, 68, 69, or 70, (ii) an RNA polymerase, and (iii) a promoter oligonucleotide, wherein the molecular torch specifically hybridizes to a sequence within a Salmonella target nucleic acid.
A kit for use in detecting Salmonella in a sample, said kit comprising (i) a molecular torch oligonucleotide comprising the nucleotide sequence consisting essentially of SEQ ID NO:
- 66, 67, 68, 69, or 70 wherein the molecular torch specifically hybridizes to a sequence within a Salmonella target nucleic acid, (ii) an RNA polymerase, and (iii) a promoter oligonucleotide for isothermally amplifying the Salmonella target nucleic acid.
Dependencies are inferred from the claim text.