Covalent tethering of functional groups to proteins
AssigneePromega Corporation
InventorsKeith V. Wood, Georgyi V. Los, Robert F. Bulleit, Dieter Klaubert, Mark McDougall, Chad Zimprich
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Abstract
A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
Claims15
A composition comprising a dehalogenase substrate of the formula R-linker-A-X, wherein R is a fluorogenic or luminogenic molecule, A-X is a substrate for said dehalogenase, X is a halogen, and the linker is a group that separates R and A;
- wherein R, linker, A, and X are covalently linked.
Dependencies are inferred from the claim text.