Biosynthesis of forskolin and related compounds
AssigneesUniversity of Copenhagen, Danmarks Tekniske Universitet, Evolva SA
InventorsBjörn Hamberger, Birger Lindberg Møller, Eirini Pateraki, Johan Andersen-Ranberg, Niels Bjerg Jensen
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Abstract
The invention described materials and methods for producing oxidized 13R-MO, such as forskolin. In particular, the invention describes P450s involved in oxidation of 13R-MO including CYP76AH8, CYP76AH11, CYP76AH15, CYP76AH17, CYP71D381 and CYP76AH9. Host organisms expressing one or more of these P450s are useful in the production of oxidized 13R-MO.
Claims9
A host organism, comprising a heterologous nucleic acid encoding an enzyme capable of catalysing hydroxylation of 13R-manoyl oxide (13R-MO) and/or an oxidised 13R-MO derivative at the 11-position, wherein the oxidised 13R-MO carries a —H at the 11-position;
- and/or catalysing oxidation of a hydroxyl group to form an oxo-group at the 11-position of 11-hydroxyl-13R-MO and/or an oxidised 11-hydroxyl-13R-MO, wherein the enzyme comprises:
- (a) CYP76AH8 having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:1;
- (b) CYP76AH17 having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:10;
- (c) CYP76AH15 having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:11;
- and/or
- (d) CYP76AH11 having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.