Recombinant microorganism for the production of useful metabolites
AssigneeScientist of Fortune, S.A.
InventorPhilippe Marliere
Life of this patent
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Abstract
Described are recombinant microorganisms characterized by having phosphoketolase activity, having a diminished or inactivated Embden-Meyerhof-Parnas pathway (EMPP) by inactivation of the gene(s) encoding phosphofructokinase or by reducing phosphofructokinase activity as compared to a non-modified microorganism and having a diminished or inactivated oxidative branch of the pentose phosphate pathway (PPP) by inactivation of the gene(s) encoding glucose-6-phosphate dehydrogenase or by reducing glucose-6-phosphate dehydrogenase activity as compared to a non-modified microorganism. These microorganisms can be used for the production of useful metabolites such as acetone, isobutene or propene.
Claims42
A genetically modified prokaryotic microorganism comprising the following characteristics:
- (A) increased phosphoketolase activity as compared to a non-genetically modified microorganism;
- and
- (B) (1) diminished or inactivated phosphofructokinase activity as compared to a non-genetically modified microorganism;
- or
- (2) not possessing phosphofructokinase activity;
- and
- (C)(1) diminished or inactivated glucose-6-phosphate dehydrogenase activity as compared to a non-genetically modified microorganism;
- or
- (2) not possessing glucose-6-phosphate dehydrogenase activity.
A method for producing acetyl-CoA from glucose comprising:
- (a) culturing a genetically modified prokaryotic microorganism in a suitable media, wherein the prokaryotic microorganism comprises the following characteristics:
- (1) increased phosphoketolase activity as compared to a non-genetically modified microorganism;
- and
- (2) (a) diminished or inactivated phosphofructokinase activity as compared to a non-genetically modified microorganism;
- or
- (b) not possessing phosphofructokinase activity;
- and
- (3) (a) diminished or inactivated glucose-6-phosphate dehydrogenase activity as compared to a non-genetically modified microorganism;
- or
- (b) not possessing glucose-6-phosphate dehydrogenase activity;
- and
- (b) recovering said acetyl-CoA.
A method for producing acetone, isobutene, or propene comprising:
- (a) culturing a genetically modified prokaryotic microorganism in a suitable media, wherein the prokaryotic microorganism comprises:
- (1) increased phosphoketolase activity as compared to a non-genetically modified microorganism;
- and
- (2) (a) diminished or inactivated phosphofructokinase activity as compared to a non-genetically modified microorganism;
- or
- (b) not possessing phosphofructokinase activity;
- and
- (3) (a) diminished or inactivated glucose-6-phosphate dehydrogenase activity as compared to a non-genetically modified microorganism;
- or
- (b) not possessing glucose-6-phosphate dehydrogenase activity;
- and
- (b) recovering said acetone, isobutene, or propene from the culture medium.
Dependencies are inferred from the claim text.