Altered host cell pathway for improved ethanol production
AssigneeDanisco US Inc.
InventorsMiasnikov Andrei, Jeffrey W. Munos
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Abstract
A recombinant yeast cell, fermentation compositions, and methods of use thereof are provided. The recombinant yeast cell includes at least one heterologous nucleic acid encoding one or more polypeptide having phosphoketolase activity; phosphotransacetylase activity; and/or acetylating acetaldehyde dehydrogenase activity, wherein the cell does not include a heterologous modified xylose reductase gene, and wherein the cell is capable of increased biochemical end product production in a fermentation process when compared to a parent yeast cell.
Claims12
A method for producing ethanol from glucose, the method comprising culturing a recombinant yeast cell in a fermentation process employing glucose feedstock, the recombinant yeast cell comprising at least one heterologous nucleic acid encoding polypeptides having:
- i) phosphoketolase activity;
- ii) phosphotransacetylase activity;
- and
- iii) acetylating acetaldehyde dehydrogenase activity,
- wherein said cell does not comprise a heterologous modified xylose reductase gene,
- wherein said cell is capable of increased ethanol production from glucose in a fermentation process when compared to the yeast cell without the at least one heterologous nucleic acid, and
- wherein the polypeptide having phosphoketolase activity has the amino acid of SEQ ID NO:
- 57, the polypeptide having acetylating dehydrogenase activity has the amino acid of SEQ ID NO:
- 32, and the polypeptide having phophotransacetylase activity is the phophotransacetylase from Lactobacillus plantarum.
Dependencies are inferred from the claim text.