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US 10,240,167 B2Granted
CRISPR enabled multiplexed genome engineering
AssigneeInscripta, Inc.
InventorsRyan T. Gill, Andrew Garst
Life of this patent
Estimating the term…
Abstract
Described herein are methods and vectors for rational, multiplexed manipulation of chromosomes within open reading frames (e.g., in protein libraries) or any segment of a chromosome in a cell or population of cells, in which various CRISPR systems are used.
Claims34
A library of at least two vectors, each vector comprising:
- (i) a nucleic acid encoding a guide RNA (gRNA) comprising a region complementary to a target region of a nucleic acid in one or more cells covalently linked to
- (ii) an editing cassette comprising
- (a) a region homologous to the target region in the one or more cells with a mutation of at least one nucleotide relative to the target region in the one or more cells, and
- (b) a protospacer adjacent motif (PAM) mutation;
- wherein the at least two vectors have different mutations relative to the target region in the one or more cells.
2 more elements
A library comprising a plurality vectors, wherein each vector comprises:
- (i) an editing cassette comprising:
- (a) a region homologous to a target region in one or more cells with a mutation of at least one nucleotide relative to the target region in the one or more cells, and
- (b) a first protospacer adjacent motif (PAM) mutation;
- and
- (ii) a nucleic acid encoding a guide RNA (gRNA) complementary to the target region of the one or more cells, wherein the editing cassette is covalently linked to the nucleic acid encoding the guide RNA, and wherein the plurality of vectors have different mutations relative to the target region in the one or more cells.
2 more elements
Dependencies are inferred from the claim text.