Compositions and methods for silencing gene expression
AssigneeA.B. Seeds Ltd.
InventorsAmir Avniel, Efrat Lidor-Nili, Rudy Maor, Ofir Meir, Orly Noivirt-Brik, Osnat Yanai-Azulay
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Abstract
A method of introducing naked dsRNA into a seed is provided. The method comprising contacting the seed with the naked dsRNA under conditions which allow penetration of the dsRNA into the seed, thereby introducing the dsRNA into the seed.
Claims16
A method of introducing naked dsRNA into a tomato seed, the method comprising shaking said tomato seed in a solution comprising a final concentration of 0.05-100 μg/μL of said naked dsRNA from 1 hour up to 60 hours, thereby introducing said naked dsRNA into said tomato seed, wherein said naked dsRNA is not associated with a cationic oligopeptide.
A method of down-regulating expression of a target gene, the method comprising:
- (a) shaking a tomato seed a solution comprising a final concentration of 0.05-100 μg/μL of a naked dsRNA from 1 hour up to 60 hours, thereby introducing said naked dsRNA into said tomato seed, wherein said naked dsRNA down regulates expression of said target gene of said tomato seed, and wherein said naked dsRNA is not associated with a cationic oligopeptide;
- and optionally
- (b) generating a tomato plant from said tomato seed.
Dependencies are inferred from the claim text.