Compositions and methods for silencing gene expression
AssigneeA.B. Seeds Ltd.
InventorsAmir Avniel, Efrat Lidor-Nili, Rudy Maor, Ofir Meir, Orly Noivirt-Brik, Osnat Yanai-Azulay
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Abstract
A method of introducing naked dsRNA into a seed is provided. The method comprising contacting the seed with the naked dsRNA under conditions which allow penetration of the dsRNA into the seed, thereby introducing the dsRNA into the seed.
Claims22
A method of introducing into a seed naked dsRNA that is capable of down-regulating a target gene, the method comprising shaking the seed in a solution comprising a final concentration of 0.013-100 μg/μL of the naked dsRNA from 1 hour up to 60 hours, thereby introducing the dsRNA into the seed, wherein the naked dsRNA is not associated with a cationic oligopeptide.
A method of down-regulating expression of a target gene, the method comprising:
- (a) shaking a seed in a solution comprising a final concentration of 0.013-100 μg/μL of a naked dsRNA from 1 hour up to 60 hours, wherein the naked dsRNA down-regulates expression of the target gene, and wherein the naked dsRNA is not associated with a cationic oligopeptide;
- and optionally
- (b) generating a plant of the seed.
Dependencies are inferred from the claim text.