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US 10,240,159 B2Granted
Protease deficient filamentous fungal cells and methods of use thereof
AssigneeGlykos Finland Oy
InventorsChristopher Landowski, Anne Huuskonen, Juhani Saarinen, Ann Westerholm-Parvinen, Anne Kanerva, Jari Natunen, Anna-Liisa Hanninen, Noora Salovuori, Merja Penttila, Markku Saloheimo
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Abstract
The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.
Claims15
A Trichoderma or Myceliophthora cell comprising at least three endogenous proteases having reduced or eliminated activity, wherein:
- a) nucleic acids encoding the proteases are modified to reduce or eliminate expression of the proteases and further comprising a recombinant polynucleotide encoding a mammalian antibody;
- b) at least two of the proteases having reduced or eliminated activity are selected from the group consisting of subtilisin proteases;
- and
- c) at least one of the proteases having reduced or eliminated activity is selected from the group of glutamic proteases consisting of:
- (i) a gap1 protease of SEQ ID NO:
- 118 (Trichoderma), SEQ ID NO:
- 574 (Myceliophthora) or SEQ ID NO:
- 587 (Myceliophthora), and
- (ii) a gap2 protease of SEQ ID NO:
- 129 (Trichoderma), SEQ ID NO:
- 585 (Myceliophthora) or SEQ ID NO:
- 588 (Myceliophthora).
9 more elements
Dependencies are inferred from the claim text.