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US 10,240,157 B2Granted
Diagnostic and screening methods for atopic dermatitis
AssigneeThe Regents of the University of California
InventorsSarah R. Wilson, Lydia Thé, Diana M. Bautista
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Abstract
The present disclosure provides methods for identifying agents that are candidate agents for treating atopic dermatitis. The present disclosure provides methods for diagnosing atopic dermatitis. The present disclosure provides compositions and methods for treating atopic dermatitis.
Claims19
A method of identifying a candidate agent for treating atopic dermatitis, the method comprising:
- a) contacting a test agent in vitro with a cell that expresses a Protease Activated Receptor 2 (PAR2) polypeptide and an ORAI1 polypeptide, wherein the PAR2 polypeptide comprises an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:21, and wherein the ORAI1 polypeptide comprises an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 5;
- b) assaying the level of one or more of an ORAI1 gene product, a STIM1 gene product, and an NFAT1 gene product in the cell;
- and c) identifying a test agent that reduces the level of one or more of an ORAI1 gene product, a STIM1 gene product, and an NFAT1 gene product in the cell, compared to a control cell not contacted with the test agent, as a candidate agent for treating atopic dermatitis.
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Dependencies are inferred from the claim text.