US 10,240,143 B2Granted

Radiofrequency device for increasing intracellular bioactive substance and plant cell culture method using the same

AssigneeBio-Fd&C Co., Ltd.

InventorsSang-Hyun Moh, Jeong-Hun Lee, Hyo-Hyun Seo, Dong-Sun Shin, Moon-Jin Cho, Hyo-Seok Kang, Hae-Soo Jung, Hyun-Jin Oh, Yeong-Wook Yu, Ji-Hong Moh, Mun-Seong Heo, Ser-Jong Yoo, Hyun-E Lee, Jin-Hyeong Lee, Yu-Ri Lee, Eun-Ae Kim, Seung-Jun Lee

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Abstract

There are provided a radiofrequency device for increasing amount of a bioactive substance in a plant cell and a plant cell culture method for increasing amount of useful intracellular secondary metabolites by using the radiofrequency device. The cell culture method of the present invention makes it possible to increase specific secondary metabolites such as daidzein, equol, and the like in a cell and thus can be used for development into various medicines, agricultural pesticides, spices, pigments, food additives, and cosmetics containing bioactive substances. Further, the cell culture method of the present invention improves conventional cell culture methods limitedly used for specific cells or specific metabolites for increasing amount of intracellular bioactive substances and thus can be widely applied to production of cells and secondary metabolites.

Claims4

Dependent claims

A soybean placenta culture method for increasing amount of intracellular bioactive substances, the method comprising:

(a1) a callus inducing step of culturing the soybean placenta in an MS (Murashige and Skoog) medium containing IAA (indole acetic acid), BAP (6-benzylaminopurine), sucrose, and gelite for 2 to 5 weeks for inducing a callus from a germinated plant;
(a2) an adventitious root inducing step of culturing the callus in an MS medium containing IBA (indole-3-butyric acid), MES monohydrate(2-(N-morpholino), benzyladenine, sucrose, and gelite for 2 to 5 weeks for inducing an adventitious root from the callus;
and
(b) a radiofrequency processing step of processing the cultured soybean placenta with a radiofrequency for increasing amount of an intracellular bioactive substance in the plant cell,
wherein the radiofrequency is used for processing is in a range of 240 to 270 kHz,
wherein the radiofrequency is used for processing 3 times every 2 to 10 minutes per day repeatedly for 5 to 15 days,
wherein the intracellular bioactive substances are daidzein and equol.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/227,886Filed 3 Aug 2016 · division
Priority: KR 10-2012-01270179 Nov 2012
Published: US 2017/0029803 A12 Feb 2017
Granted: 26 Mar 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12N 13/00
Treatment of microorganisms or enzymes with electrical or wave energy
A01H 4/002
Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor: culture media for tissue culture
C12M 35/04
Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for…:…
C12N 5/04
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor: plant cells…

Family

Application14/076,139Parent of this division · filed 8 Nov 2013
US 2017/0029803 A1Pre-grant publication · published 2 Feb 2017
US 9,464,282 B2Parent of this division
US 2014/0335587 A1Published application
US 10,240,143 B2This patent

From the US filing history. Foreign family members aren’t listed.