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AssigneeMiyoshi Oil & Fat Co., Ltd.
InventorsKotaro Kaneko, Nobuhiro Kaneko, Koji Kawai
Estimating the term…
There is provided a biocatalyst solvent which is capable of dissolving a biocatalyst in a liquid state while maintaining the activity of the biocatalyst at a high concentration from a low temperature to a high temperature, and is capable of storing the biocatalyst for a long time period; and a biocatalyst solution using the biocatalyst solvent. Disclosed is a biocatalyst solvent which consists of an ionic liquid including a quaternary ammonium cation represented by the following Formula (I) and an anion: [Chemical Formula 1] N+[Ra]n[Rb]4-n (I) wherein Ra's each independently represent a hydroxyalkyl group having one or more hydroxyl groups and an a linear or branched alkyl moiety having 1 to 10 carbon atoms, in which the alkyl moiety may contain an oxygen atom; a carboxyalkyl group having one or more carboxyl groups and a linear or branched alkyl moiety having 1 to 10 carbon atoms, in which the alkyl moiety may contain an oxygen atom; or a hydroxycarboxyalkyl group having one or more hydroxyl groups, one or more carboxyl groups and a linear or branched alkyl moiety having 1 to 10 carbon atoms, in which the alkyl moiety may contain an oxygen atom; Rb's each independently represent a hydrogen atom, or a linear or branched alkyl group having 1 to 5 carbon atoms; and n represents an integer from 1 to 4.
A method for stabilizing enzyme activity during storage in a solvent comprising:
Dependencies are inferred from the claim text.
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Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: stabilising an…
And acyclic: with only one hydroxy group and one amino group bound to the carbon skeleton
And acyclic: with one amino group and at least two hydroxy groups bound to the carbon skeleton
The nitrogen atom of the amino group being further bound to acyclic carbon atoms or to carbon atoms of rings other than six-membered aromatic…
About 9 years of term left · ends 21 Oct 2035 (estimated)
The present invention relates to a biocatalyst solvent that can dissolve a biocatalyst in a state of retaining the biocatalytic activity, and a biocatalyst solution containing the solvent and a biocatalyst.
Many of biocatalysts such as enzymes and yeasts are prone to undergo destruction of the molecular conformation and deterioration of the activity, that is, catalytic ability, under the influence of temperature, pH, solvent, or intermolecular electrostatic repulsion. Therefore, when a biocatalyst is stored or utilized in a biocatalytic reaction, it is necessary to maintain the conformation of an active site and the conformation of amino acid residues. Regarding a method for long-term storage of a biocatalyst, there are known a freeze drying method of storing a biocatalyst in a powdered form, and a freeze preservation method of storing a biocatalyst by dissolving the biocatalyst in a solution under cryogenic conditions at a low concentration.
Generally, storage as a solution is desirable in view of the convenience of operation; however, in the case of a freeze preservation method, special apparatuses are required, and also, there are a problem that since ice is produced at the time of freezing, the conformation of a biocatalyst is destroyed, and a problem that when a frozen solution is used after thawing, the structure of the biocatalyst changes, and the activity is deteriorated. Furthermore, the storage concentration is, for example, in the case of urease or catalase, generally as low as about 1 to 3 mg/mL, and efficient preservation is difficult.
Hereinafter, the present invention will be described in detail.
The biocatalyst solvent of the present invention may be an anhydrous ionic liquid or a hydrous ionic liquid that has absorbed the moisture in air, and consists of an ionic liquid containing a quaternary ammonium cation represented by Formula (I) and an anion.
In Formula (I), Ra's of the quaternary ammonium cation each independently represent a hydroxyalkyl group having one or more hydroxyl groups and having a linear or branched alkyl moiety having 1 to 10 carbon atoms, in which the alkyl moiety may contain an oxygen atom; a carboxyalkyl group having one or more carboxyl groups and having a linear or branched alkyl moiety (not including the carbon atom of the carboxyl group) having 1 to 10 carbon atoms, in which the alkyl moiety may contain an oxygen atom; or a hydroxycarboxyalkyl group having one or more hydroxyl groups, one or more carboxyl groups and a linear or branched alkyl moiety (not including the carbon atom of the carboxyl group) having 1 to 10 carbon atoms, in which the alkyl moiety may contain an oxygen atom.