Expression system for psicose epimerase and production for psicose using the same

A gene expression cassette, producing a psicose epimerase in Corynebacterium sp., and comprising:

• a nucleotide sequence encoding the psicose epimerase;
• and
• a regulating sequence being operably connected to the nucleotide sequence in the upstream regulating the expression of the nucleotide sequence in Corynebacterium sp, and comprising a promoter, a ribosome binding site (RBS) sequence and a first spacer sequence in the direction of 5′ to 3′,
• wherein the promoter includes the nucleotide sequence of SEQ ID NO:
• 1,
• the ribosome binding site (RBS) sequence is a nucleotide sequence in a size of 7 to 20 bases including the nucleotide sequence of SEQ ID NO:
• 2, and
• the first spacer sequence is selected from the group consisting of the nucleotide sequences of SEQ ID NO:
• 3 to SEQ ID NO:
• 6,
• wherein the regulating sequence further comprises a second RBS sequence which is connected to 3′-end of the first spacer directly or via a linker sequence in a length of 5 to 100 bases,
• wherein the second RBS sequence is a nucleotide sequence in a size of 7 to 20 bases including the nucleotide sequence of SEQ ID NO:
• 2,
• wherein the regulating sequence further comprises a second spacer sequence selected from the group consisting of the nucleotide sequences of SEQ ID NO:
• 7 to SEQ ID NO:
• 11, wherein the second spacer is connected to 3′-end of the second RBS.