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US 10,240,135 B2Granted
Variants and compositions comprising variants with high stability in presence of a chelating agent
AssigneeNovozymes A/S
InventorsAllan Svendsen, Annette Helle Johansen, Mads Bjoernvad, Frank W. Rasmussen, Michael Skjoet, Signe Eskildsen Larsen, Jens Oebro, Svend Kaasgaard, Lars Beier
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Abstract
The present invention relates to variants of an alpha-amylase having improved stability to chelating agents relative to its parent enzyme, compositions comprising the variants, nucleic acids encoding the variants, methods of producing the variants, and methods for using the variants.
Claims25
A variant of a parent alpha-amylase comprising:
- at least two deletions in amino acid positions 181, 182, 183, or 184;
- a substitution at amino acid position 206, and a substitution at one or more amino acid positions selected from the group consisting of 116, 118, 129, 133, 142, 146, 147, 149, 151, 152, 169, 174, 186, 235, 244, 303, 320, 339, 359, 418, 431, 434, 447 and 458, wherein
- (a) the variant has alpha-amylase activity;
- and
- (b) each amino acid position corresponds to a position set forth in SEQ ID NO:
- 6.
3 more elements
A composition comprising a variant of a parent alpha-amylase, wherein the variant comprises a substitution corresponding to amino acid position 206 in SEQ ID NO:
- 6, and further comprises at least one chelating agent, and wherein said chelating agent at a concentration below 10 mM reduces the concentration of free calcium ions from 2.0 mM to 0.10 mM when measured at 21° C. and pH 8.0.
Dependencies are inferred from the claim text.