Method for preparing induced paraxial mesoderm progenitor (IPAM) cells and their use
AssigneesInserm (Institut National De La Sante Et De La Recherche Medicale), Cnrs (Centre National De La Recherche Scientifique), Universite De Strasbourg, Association Francaise Contre Le Myopathies
InventorsOlivier Pourquie, Jérôme Chal
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Abstract
The present invention relates to an ex vivo method for preparing induced paraxial mesoderm progenitor (iPAM) cells, said method comprising the step of culturing pluripotent cells in an appropriate culture medium comprising an effective amount of an activator of the Wnt signaling pathway and an effective amount of an inhibitor of the Bone Morphogenetic Protein (BMP) signaling pathway.
Claims16
An ex vivo method for preparing a cell population comprising induced human paraxial mesoderm progenitor (iPAM) cells, the method comprising:
- culturing human pluripotent cells in a basal culture medium comprising an effective amount of a R-spondin protein or a GSK-3β inhibitor, wherein the culturing results in the production of a human cell population comprising iPAM cells expressing Msgn1 and Tbx6.
An ex vivo method for preparing a cell population comprising induced human paraxial mesoderm progenitor (iPAM) cells, the method comprising:
- culturing human pluripotent cells selected from the group consisting of human embryonic stem cells or human induced pluripotent stem (iPS) cells, in a basal culture medium comprising an effective amount of a BMP4 inhibitor, and an effective amount of a R-spondin protein or a GSK-3β inhibitor, wherein the culturing results in the production of a human cell population comprising iPAM cells expressing Msgn1 and Tbx6.
Dependencies are inferred from the claim text.