Cell line overexpressing human CD303 antigen
AssigneeLaboratoire Francais Du Fractionnement Et Des Biotechnologies
InventorsNathalie Fournier, Christophe De Romeuf
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Abstract
The invention relates to a cell line expressing the gamma chain of the FcεRI receptor and human CD303 antigen, characterized in that said cell line is transfected in a stable manner by an expression vector comprising a nucleic acid molecule coding for human CD303 antigen and having strong expression of human CD303 on the surface thereof, e.g. at least 10000 molecules of human CD303 per cell, as well as a vector or vector kit that can be used to co-express the gamma chain of the FcεRI receptor and human CD303 antigen, and different uses of the cell line of the invention.
Claims14
A cell line expressing the gamma chain of Fcε receptor I (FcεRI) and human CD303 antigen, wherein said cell line is stably transfected with an expression vector comprising a nucleic acid molecule encoding human CD303 antigen and has high expression of human CD303 on its surface, wherein said cell line has at least 10,000 human CD303 molecules per cell and said cell line is:
- a) a cell line of human plasmacytoid dendritic cells (pDCs) stably transfected with an expression vector comprising a nucleic acid molecule encoding human CD303 antigen;
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- b) a human lymphocyte cell line, stably transfected with an expression vector comprising a first nucleic acid molecule encoding human CD303 antigen and a second nucleic acid molecule encoding the gamma chain of human FcεRI, or with two expression vectors each comprising a nucleic acid molecule encoding human CD303 antigen and the gamma chain of human FcεRI, respectively.
Dependencies are inferred from the claim text.