Monoclonal antibodies directed against trimeric forms of the HIV-1 envelope glycoprotein with broad and potent neutralizing activity

A method of inhibiting HIV in a host comprising administering to the host a non-naturally occurring monoclonal anti-HIV antibody, designated 1443 C16 (PG16) or antigen binding portion thereof comprising (a) a light chain variable region comprising CDRs having the amino acid sequences of SEQ ID NOS:

• 41, 95, and 97 and (b) a heavy chain variable region comprising CDRs having the amino acid sequences of SEQ ID NOS:
• 6, 88 and 89.

A method of inhibiting HIV in a host comprising administering to the host a non-naturally occurring monoclonal anti-HIV PG9 antibody or antigen binding portion thereof comprising a light chain sequence of SEQ ID NO:

• 30 and a heavy chain sequence of SEQ ID NO:
• 28.

A method of inhibiting HIV in a host comprising administering to the host a non-naturally occurring monoclonal anti-HIV PG16 antibody or antigen binding portion thereof comprising a light chain sequence of SEQ ID NO:

• 14 and a heavy chain sequence of SEQ ID NO:
• 12.

An expression vector that encodes and stably expresses in vivo an antibody comprising each of a light chain sequence of SEQ ID NO:

• 30 and a heavy chain sequence of SEQ ID NO:
• 28.

An expression vector that encodes and stably expresses in vivo an antibody comprising each of a light chain sequence of SEQ ID NO:

• 14 and a heavy chain sequence of SEQ ID NO:
• 12.

An expression vector that encodes and stably expresses in vivo an antibody comprising (a) a light chain variable region comprising complementarity determining regions (CDRs) having the amino acid sequences of SEQ ID NOS:

• 45, 126 and 127 and (b) a heavy chain variable region comprising CDRs having the amino acid sequences of SEQ ID NOS:
• 7, 123 and 124.

An expression vector that encodes and stably expresses in vivo an antibody comprising (a) a light chain variable region comprising CDRs having the amino acid sequences of SEQ ID NOS:

• 41, 95, and 97 and (b) a heavy chain variable region comprising CDRs having the amino acid sequences of SEQ ID NOS:
• 6, 88 and 89.