Method for identifying novel minor histocompatibility antigens

A method of treating cancer, said method comprising administering to a subject in need thereof an effective amount of CD8 T lymphocytes recognizing:

• (a) a MHC class I molecule of the HLA-B*0801 allele loaded with a peptide of 8 to 12 amino acids comprising (i) one of the following sequences:
• ELQEKFLSL (SEQ ID NO:15) or ELQEKFSSL (SEQ ID NO:16);
• and/or (ii) one of the following sequences:
• SLFFRKVPF (SEQ ID NO:19) or SLFFRKVAF (SEQ ID NO:20), if said subject expresses said MHC class I molecule of the HLA-B*0801 allele;
• (b) a MHC class I molecule of the HLA-B*4403 allele loaded with a peptide of 10 to 12 amino acids comprising one the following sequences:
• QELDGVFQKL (SEQ ID NO:17) or QELDRVFQKL (SEQ ID NO:18), if said subject expresses said MHC class I molecule of the HLA-B*4403 allele;
• and/or
• (c) a MHC class I molecule of the HLA-A*0301 allele loaded with a peptide of 9 to 12 amino acids comprising (i) one of the following sequences:
• RVSLPTSPG (SEQ ID NO:25) or RVSLPTSPR (SEQ ID NO:26);
• (ii) one of the following sequences:
• SVLKPGNSK (SEQ ID NO:21) or TVLKPGNSK (SEQ ID NO:22);
• (iii) one of the following sequences:
• AMYDKGPFRSK (SEQ ID NO:23) or AMYDKGPFWSK (SEQ ID NO:24);
• and/or (iv) one of the following sequences:
• VMGNPGTFK (SEQ ID NO:27) or VMGNPGTFN (SEQ ID NO:28), if said subject expresses said MHC class I molecule of the HLA-A*0301 allele.