Back to results
US 10,239,853 B1Granted
Polycyclic phosphoramidtes and their applications
AssigneeAAT Bioquest, Inc.
InventorsZhenjun Diwu, Ruogu Peng, Haitao Guo, Zhen Luo
Life of this patent
Estimating the term…
Abstract
The present invention is generally directed to fluorescent dyes useful for preparing fluorescent oligonucleotides, and the use of the fluorescent oligonucleotides for the detection, discrimination and quantification of molecular biology targets.
Claims20
A phosphoramidite having Formula 1:
- wherein R1-R6 are independently a hydrogen, an alkyl, an aryl or a heteroaryl;
- R7-R16 are independently H, an alkyl, a halogen, carboxy, an alkoxy, an aryloxy, thiol, a alkylthiol, an arylthiol, azido, nitro, nitroso, cyano, an amino, hydroxy, a phosphonyl, a sulfonyl, a carbonyl, a boronyl, an aryl, a heteroaryl or a phosphoramidite;
- X is N—R18, P—R18, O═P—R18, O═P—OR18, R18—C—R19, R18—Si—R19, S, O═S—R18 or O═S(O)—R18;
- provided that one of R7-R19 is L-O—P(O—R20)(NR21R22) wherein L is none or a linker, R18 to R22 are an alkyl, an aryl or a heteroaryl.
1 more element
A phosphoramidite having Formula 3:
- wherein R1-R6 are independently a hydrogen or an alkyl;
- R7-R16 are hydrogen, chloro, fluoro or a phosphoramidite;
- provided that at least two of R7-R16 are fluoro, and one of R7-R16 is L-O—P(O—R20)(NR21R22) wherein L is none or a linker, and R20 to R22 are an alkyl or an aryl.
A fluorescent oligonucleotide prepared from the phosphoramidite having Formula 1:
- wherein R1-R6 are independently a hydrogen, an alkyl, an aryl or a heteroaryl;
- R7-R16 are independently H, an alkyl, a halogen, an aryl, a heteroaryl or a phosphoramidite;
- X is O═P—R18, O═P—OR18, R18—C—R19 or R18—Si—R19;
- provided that one of R7-R19 is L-O—P(O—R20)(NR21R22) wherein L is none or a linker, R18 to R22 are an alkyl, an aryl or a heteroaryl.
1 more element
A fluorescent oligonucleotide prepared from the phosphoramidite having Formula 3:
- wherein R1-R6 are methyl;
- R7-R16 are hydrogen, chloro, fluoro or a phosphoramidite;
- provided that at least two of R7-R16 are fluoro, and one of R14 and R15 is L-O—P(O—CH2CH2CN)(NR21R22) wherein R21 and R22 are isopropyl.
Dependencies are inferred from the claim text.