Methods, systems and devices for selection and generation of genome edited clones

A method of generating a clonal population of genetically modified cells in a microfluidic device comprising a sequestration pen, the method comprising:

• maintaining a first cell in the sequestration pen of the microfluidic device, wherein the first cell has undergone a genome editing process;
• expanding the first cell into a clonal population of cells;
• and
• detecting, in one or more cells of the clonal population, the presence of a first nucleic acid sequence,
• wherein at least one inner surface of the sequestration pen is a conditioned surface comprising covalently-linked molecules, each having a linking group (LG) covalently bound to the at least one inner surface and a moiety covalently bound to the linking group (LG) via a linker (L) having a linear backbone of 10 to 20 carbon atoms,
• wherein the moieties of the covalently-linked molecules include amino acid-containing polymers suitable for maintenance and/or expansion of the genome-edited cell, and
• wherein the first nucleic acid sequence indicates the presence of an on-target genome edit in the clonal population of cells.