Bone regeneration using biodegradable polymeric nanocomposite materials and applications of the same

A method of producing a biocompatible structure for bone and tissue regeneration, comprising:

• forming a plurality of first polymer layers by:
• dissolving a first polymer in a first solvent to form a first solution;
• adding first tissue forming nanoparticles to the first solution to form a second solution, wherein a first weight percentage of the first tissue forming nanoparticles to the first polymer is about 0.01-95%;
• applying the second solution to a first surface to form a first polymer film on the first surface, wherein the first tissue forming nanoparticles are dispersed in the first polymer film;
• and
• dividing the first polymer film into the plurality of first polymer layers;
• forming a plurality of second polymer layers by:
• dissolving a second polymer in a second solvent to form a third solution;
• adding second tissue forming nanoparticles to the third solution to form a fourth solution, wherein a second weight percentage of the second tissue forming nanoparticles to the second polymer is greater than the first weight percentage;
• applying the fourth solution to a second surface to form a second polymer film on the second surface, wherein the second tissue forming nanoparticles are dispersed in the second polymer film;
• and
• dividing the second polymer film into the plurality of second polymer layers;
• and
• forming the biocompatible structure by the plurality of first polymer layers, first spacer particles, the plurality of second polymer layers, second spacer particles, and a fifth solution,
• wherein the biocompatible structure includes
• the plurality of first polymer layers;
• the plurality of second polymer layers;
• the first spacer particles placed between two first polymer layers of the plurality of first polymer layers;
• and
• the second spacer particles placed between two second polymer layers of the plurality of second polymer layers;
• and
• wherein the fifth solution includes
• at least one of the first and the second tissue forming particles;
• at least one of the first polymer and the second polymer;
• and
• at least one of the first solvent and the second solvent,
• wherein the step of forming the biocompatible structure comprises:
• constructing a first base structure by stacking the plurality of first polymer layers and first spacer layers alternatively, wherein each of the first spacer layers is formed by the first spacer particles;
• constructing a second base structure on the first base structure by stacking the plurality of second polymer layers and second spacer layers alternatively, wherein each of the second spacer layers is formed by the second spacer particles;
• applying the fifth solution to the first base structure and the second base structure to form a coated structure;
• and
• adding third spacer particles to the coated structure to form the biocompatible structure.