High oleic seed oil trait in cotton varieties to elevate oleic acid content of cottonseed oil
AssigneeUniversity of North Texas
InventorsKent Chapman, Patrick Horn, Drew Sturtevant, Christopher Kennedy
Life of this patent
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Abstract
An identified 90 bp sequence insertion in the GB0331 genome disrupts the coding sequence of the FAD2-1D gene near the 3′ end of the protein coding region. This sequence is not found in reference genomes of other Gossypium species. This DNA segment can be used to track the high oleic fad-2-1D mutant allele for introgression into cotton varieties such as Pima and upland cotton varieties. This enables a non-GMO strategy to elevate the oleic acid content of commercial cottonseed oil.
Claims14
A method for obtaining and identifying cotton seeds generated to have an elevated oleic acid content, comprising:
- obtaining a cotton plant suspected of having a high oleic acid trait;
- extracting nucleic acid material from cells of the cotton plant;
- amplifying the nucleic acid material extracted from cells of the cotton plant using a primer comprising a sequence specific for an insertion in a mutated FAD2-1D allele found in the cotton plant genome, wherein the insertion in the mutated FAD2-1D allele comprises SEQ ID NO:2;
- detecting amplified mutated nucleic acid material from cells of the cotton plant, wherein detection of amplified mutated nucleic acid material identifies the cotton plant as having a high oleic acid trait;
- and
- generating cotton seeds from the cotton plant, wherein the cotton seeds have an elevated oleic acid content.
A method for obtaining and identifying a cotton plant having a high oleic acid trait, comprising:
- obtaining a cotton seed suspected of having a high oleic acid trait;
- extracting nucleic acid material from cells of the cotton seed;
- amplifying the nucleic acid material extracted from cells of the cotton seed using a primer comprising a sequence specific for an insertion in a mutated FAD2-1D allele found in the cotton seed genome, wherein the insertion in the mutated FAD2-1D allele comprises SEQ ID NO:2;
- detecting amplified mutated nucleic acid material from cells of the cotton seed, wherein detection of amplified mutated nucleic acid material identifies the cotton seed as having a high oleic acid trait;
- and
- germinating the cotton seed to produce a cotton plant, wherein the cotton plant has a high oleic acid trait.
Dependencies are inferred from the claim text.