Enzyme detection device

An enzyme detection device for use in the detection of enzyme activity in a test sample comprising:

• (i) an indicator molecule for adding to the test sample, said indicator molecule comprising
• (a) an enzyme modifiable region, which can be modified by said enzyme causing transformation of the region from an unmodified to a modified state;
• and
• (b) a detection region, which is not modified by the enzyme and can be bound by a reporter molecule irrespective of the state of modification of the enzyme modifiable region,
• (ii) a selective capture zone to receive the test sample, wherein the selective capture zone comprises selective recognition molecules capable of binding to the enzyme modifiable region of the indicator molecule selectively in one or other of the modified or unmodified states;
• and
• (iii) a detection zone to receive the test sample following contact of the test sample with the selective capture zone, wherein the detection zone is spatially separated from the selective capture zone such that exposure of the test sample to the detection zone occurs after prior exposure of the test sample to the selective capture zone and wherein only indicator molecules that do not bind to the selective recognition molecules in the selective capture zone pass into the detection zone and wherein the indicator molecule is detected at the selective capture zone and/or the detection zone;
• and wherein the device additionally comprises a reporter molecule bound or capable of binding directly to the detection region of the indicator molecule, and the binding of the reporter molecule to the indicator molecule occurs irrespective of the state of modification of the enzyme modifiable region.