Nucleotide sequences and corresponding polypeptides conferring modulated growth rate and biomass in plants grown in saline conditions
AssigneeCeres, Inc.
InventorsFasong Zhou, Kenneth A. Feldmann, Julissa Sosa
Life of this patent
Estimating the term…
Abstract
The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline conditions with respect to wild-type plants grown under similar conditions.
Claims8
A plant cell transformed with a recombinant nucleic acid molecule, wherein the recombinant nucleic acid molecule comprises:
- (a) a nucleotide sequence that encodes a protein comprising the amino acid sequence of SEQ ID NO:154;
- (b) a nucleotide sequence according to SEQ ID NO:153, wherein the nucleotide sequence encodes the protein of SEQ ID NO:154;
- or
- (c) a nucleotide sequence hybridizing to the full length complementary sequence of the nucleotide sequence as set forth in SEQ ID NO:
- 153 under high stringency conditions which comprise (i) hybridization at 42° C. in 50% formamide, 6×SSC or 6×SSPE, 0.05% Blotto or 5×Denhardt's Reagent, 100 μg/ml denatured salmon sperm DNA, 0.05% SDS and washing at 65° C. first in 2×SSC, 0.1% SDS for at least 30 min to one hour and subsequently in 0.1×SSC, 0.5% SDS for at least 30 min to one hour;
- or (ii) hybridization at 65° C. in 6×SSC or 6×SSPE, 0.05% Blotto or 5×Denhardt's Reagent, 100 μg/ml denatured salmon sperm DNA, 0.05% SDS and washing at 65° C. first in 2×SSC, 0.1% SDS for at least 30 min to one hour, and subsequently in 0.1×SSC, 0.5% SDS for at least 30 min to one hour;
- and wherein said hybridizing nucleotide sequence encodes a protein which has the functional activity of SEQ ID NO:
- 154;
- wherein said nucleotide sequence is operably linked to a heterologous promoter, wherein the protein encoded by the nucleotide sequence is expressed;
- and wherein expression of the protein encoded by said nucleotide sequence in said plant cell increases salt stress tolerance as compared to a control plant cell of the same plant cell species that has not been transformed with the recombinant nucleic acid molecule.
Dependencies are inferred from the claim text.