Engineered primate L-methioninase for therapeutic purposes
AssigneeBoard of Regents, The University of Texas System
InventorsGeorge Georgiou, Everett Stone, Wei-Cheng Lu
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Abstract
Methods and compositions relating to the engineering of an improved protein with methionine-γ-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-γ-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.
Claims18
A polypeptide comprising a variant of a native primate cystathionine-γ-lyase (CGL) enzyme, wherein the variant CGL has methionine-γ-lyase activity, comprises a sequence at least 95% identical to SEQ ID NO:
- 1, and comprises amino acid substitutions comprising E59N, R119L, and E339V of SEQ ID NO:
- 1, and at least one of the following substitutions:
- S63L, L91M, K268R, T311G, and I353S.
Dependencies are inferred from the claim text.